OC-3-VGH卵巢癌细胞简捷培养方法的研究  

Study on Cell Culture Method of Ovarian Cancer Cell Line OC-3-VGH

作　　者：刘向云[1] 杨荣富[1] 徐滢雯[1] 谢琛静[1] 王玖玖[1] 胡文娟[1] 蒋秀蓉[1] 孙祖越[1] 

机构地区：[1]上海市计划生育科学研究所,200032

出　　处：《中国计划生育学杂志》2009年第7期424-426,共3页Chinese Journal of Family Planning

摘　　要：目的:探索简捷细胞培养方法并观察该方法对癌细胞特性的影响。方法:将复苏的OC-3-VGH卵巢癌细胞株不经洗涤直接移至细胞培养瓶,加入10ml RPMI-1640培养液,放入培养箱,余同传统方法,观察细胞生长情况;取细胞悬液,以细胞数4×106/ml,0.2ml/只接种至BALB/c雌性裸小鼠皮下,2个月后处死,取肿瘤组织制片,并与传统培养方法结果进行比较。结果:两种方法培养的细胞均生长活跃,皮下接种7d左右,裸鼠长出肿瘤,组织学检查未见明显差异。结论:改良细胞培养方法减少了传统方法的繁琐步骤,方便细胞培养。Objective： To explore a modified cell culture method and observe its influences on characteristics of cell line OC-3-VGH of ovarian cancer. Methods： Resuscitated cells of ovarian cancer cell line OC-3-VGH were moved to cell culture bottle directly without washing, and put into ineubaton after adding 10ml RPMI - 1640 nutrient solution. The other processes were consistent with the traditional method, and the situation of cell growth was observed. Cell suspension （4×10^6/ml） was taken out and inoculated to BALB/c female nude mice（0.2ml per mouse）. All mice were executed after 2 months, and tumor tissues were taken out. Tissue sections were prepared and compared with the traditional culture method. Results： Active cell growths were presented in the 2 cell culture methods. Tumor tissues were observed almost at the 7th day after hypodermic inoculation. And there were no significant differences in histological examination. Conclusion： The modified cell culture method of this study is more convenient for cell culture than traditional method with its simple procedures.

关 键 词：细胞培养 癌细胞株 改良培养法 

分 类 号：R737.31[医药卫生—肿瘤]