核黄素-月桂酸酯对化疗大鼠小肠黏膜的保护作用  

Protective effects of riboflavin laurateon on small intestinal mucosa against chemotherapeutic injury in rats

作　　者：张慧[1] 王彦博[2] 王强[3] 任建平[4] 徐琪寿[4] 

机构地区：[1]武警黄金指挥部门诊部,北京100085 [2]武警北京总队医院,北京100024 [3]解放军总医院第二附属医院普外科,北京100091 [4]军事医学科学院放射与辐射医学研究所,北京100102

出　　处：《感染．炎症．修复》2009年第2期85-88,F0003,共5页Infection Inflammation Repair

摘　　要：目的：探讨核黄素-月桂酸酯（RFL）对化疗后小肠黏膜的保护作用。方法：70只Wistar大鼠,体重210-250g,随机分4组：正常对照组（n=10）、甲氨喋呤（MTX）组（n=20）、RFL预防组（n=20）和RFL预防＋甲酰四氢叶酸钙（CF）组（n=20）。化疗药选用MTX。正常对照组每日皮下注射生理盐水1ml,共3d,MTX组皮下注射MTX3.0mg·kg^-1·d^-1,连续3d;RFL预防组于化疗前24h肌肉注射RFL25mg/kg,给药1次;RFL预防＋CF组于化疗前24h肌肉注射RFL25mg/kg,于化疗后24h皮下注射CF15mg·kg^-1·d^-1,共3d。观察大鼠化疗后6d和10d小肠重量、小肠绒毛高度以及小肠黏膜组织病理学的改变。结果：化疗第6天,RFL预防用药两组小肠重量[（5.85±0.67）g和（7.93±0.72）g]重于MTX组[（5.01±0.57）g,P〈0.05和P〈0.01];小肠绒毛高度[（274.9±50.2）μm和（304.5±60.7）μm]显著高于MTX组[（171.7±30.1）μm,P〈0.01];MTX对照组小肠黏膜充血、炎细胞浸润等损伤病变明显,RFL用药两组病理学变化均较MTX组轻。化疗第10天,RFL预防用药两组小肠绒毛高度仍显著高于MTX组（P〈0.01和P〈0.05）,小肠重量则无明显差异,且大部分动物的肠黏膜损伤病变已经恢复,仅MTX组个别动物的肠黏膜仍可见轻微病变,如绒毛相互融合。结论RFL能预防性对抗氧化性损伤,减少化疗对小肠黏膜的损害。Objective： To study the protective effects of riboflavin laurate（RFL） on small intestinal mucosa against chemotherapeutic injury in rats. Methods：Seventy rats,weighing 210-250 g, were randomly divided into four groups. Group A was normal control （n= 10）, group B was methotrexate （MTX） control （n=20）, group C was RFL pretreatment before MTX injection （RBF,n= 20）, group D was RFL pretreatment group before MTX injection and subcutaneous calcium folinate （CF） injection 24 hours after MTX injection （RFL＋CF, n= 20）. The rats in groups A were subcutaneously injected with 1 ml of normal saline on day 1, 2 and 3. All rats in groups 13, C,D were subcutaneously injected with the chemotherapeutic drug MTX （3.0 mg · kg^-1 · d^-1） on days 1, 2 and 3. RFL（25 mg/kg） was intramuscularly injected once 24 hours before MTX injection in group B and C. CF（15 mg · kg^-1 · d^-1） was subcutaneously injected 24 hours after MTX injection in group D. Rats were sacrificed on days 6 and 10 after MTX injection. The weight of small intestine, villus height in the intestinal mucosa, changes in pathological changes in small intestinal tissue was examined with light and electron microscopy. Results：On day 6, the weight of small intestinal of RFL and RFL ＋CF [（5. 85±0. 67）g and（7.93±0.72）g] were higher than MTX control [ （5.01 ± 0.57） g, P〈0.05 and P〈0. 01] ; the height of villi in RFL and RFL＋ CF [（274.9 ± 50.2） μm and（304.5±60.7）μm] was obviously higher than MTX control [（171.7±30.1）μm,P〈0. 011. Small bowel injury was severe in MTX group, showing engorgement in jejunal mucosa, with infiltration of inflammatory ceils. The morphologic changes in small intestinal tissue of RFL and RFL＋CF were milder than MTX control. On day 10, the villus height in RFL and RFL＋CF was also obviously higher than MTX control （P〈0. 01 and P〈0. 05） ; but the difference was not significant among MTX, RFL and RFL＋CF groups in the weight of small intestine. The intes

关 键 词：核黄素 化疗 抗氧化 小肠黏膜炎 

分 类 号：R-332[医药卫生] R364.5