坐标定位划痕法在研究“细胞创面”愈合率中的应用  

The application of rectangular coordinate positioning scraping in evaluation of healing rate of “cell-wound” healing

作　　者：李富[1] 刘文忠[1] 雷永红[2] 张翠萍[2] 蔡飒[2] 孙同柱[2] 陈小波[2] 包晓霞[2] 

机构地区：[1]内蒙古医学院基础医学院人体解剖学教研室,内蒙古呼和浩特010059 [2]解放军总医院第一附属医院全军创伤修复重点实验室,北京100037

出　　处：《感染．炎症．修复》2009年第2期93-94,共2页Infection Inflammation Repair

基　　金：国家重点基础研究发展规划资助项目(2005CB522603);全军"十一五"课题(069116);国家自然科学基金资助项目(30400172)

摘　　要：目的:改良传统"细胞创面"模型的制备方法,提高对"细胞创面"愈合率的统计的精确性。方法:在6孔板每一孔外底面用刀尖划上横纵坐标轴,用绿色记号笔在横轴的上、下方平行于横轴各画5条等距离的绿带。接种目的细胞,待其长满内表面时用1000μl微量移液器的吸头在纵轴的左、右平行于纵轴各划2条等距离的细胞缺损带。在观察时间点观察绿带与细胞缺损带重叠形成的"矩形区域"的宽度或面积,计算愈合率。结果:可以特定追踪观察每个"矩形区域"内"细胞创面"愈合率情况,且"矩形区域"的例数可以符合统计学要求。结论:坐标划痕法是一种较精确统计"细胞创面"愈合率可行的方法。Objective：To investigate how to improve the exactness of evaluation of ＂cells wound healing rate＂, and to improve the traditional method of scraping. Methods： Rectangular coordinate axis was inscribed on the ex ternal undersurface of every well of 6-well plate. And, 5 parallel equidistance green zones were painted on the two sides of X-axis. Then, the cells were implanted in the 6-well plate. When the wells were fully grown, two parallel equidistance cell defect zones could be scraped on the two side of Y-axis by the suction head of 1 000 μl-transferpipel. Thus every overlapping area of green zones and cells defect zones was rectangular area with differential coordinates. The width of the rectanguler areas were measured in definite intervals, and the ＂cell wound healing rate＂ was calculated. Results： The cell-wound healing rate of every target rectangular area can be observed continuously, and the number of target rectangular areas could meet the requisites of statistics. Conclusion： Rectangular coordinate positioning scraping is a precise and feasible method to investigate the cell wound healing rate.

关 键 词：坐标定位划痕法 “细胞创面”愈合率 矩形区域 

分 类 号：R573.1[医药卫生—消化系统] R318.08[医药卫生—内科学]