重症肌无力患者FOXP3表达变化与体液免疫异常的相关性  

The Correlation between the Expression of FOXP3 and Humoral Immunity in Myasthenia Gravis Patients

作　　者：杨欢[1] 肖波[1] 邓茂林[1] 赵海婷[1] 舒孔亮[1] 黄慧芬[1] 

机构地区：[1]湖南省长沙市中南大学湘雅医院神经内科,湖南长沙410008

出　　处：《中国神经免疫学和神经病学杂志》2009年第4期235-240,共6页Chinese Journal of Neuroimmunology and Neurology

基　　金：国家自然科学基金资助项目(30570639)

摘　　要：目的探讨重症肌无力(MG)患者外周血叉头样转录因子P3(FOXP3)表达变化与体液免疫异常的关系。方法利用RT-PCR技术检测41例MG患者和20名体检健康者外周血单个核细胞FOXP3、B淋巴细胞激活因子(BLyS)、补体调节因子膜反应性溶解抑制物(CD59)和衰变加速因子(DAF)的mRNA表达,用ELISA法检测外周血清转化生长因子(TGF-β1)、抗乙酰胆碱受体抗体(anti-AChR-IgG)水平,并进一步分析其间的关系。结果(1)MG患者组外周血FOXP3、CD59、DAF的mRNA表达及血清TGF-β1水平明显低于健康对照组,BlySmRNA表达水平明显高于健康对照组(均P<0.01);(2)外周血FOXP3、BLyS、CD59、DAF的mRNA表达及血清TGF-β1水平,在眼肌型与全身型MG患者间差异无统计学意义;(3)41例MG患者中血清抗AChR-IgG阳性30例,眼肌型(18例)和全身型(12例)血清抗AChR-IgG水平差异无统计学意义(P>0.05);(4)MG组外周血单个核细胞FOXP3 mRNA表达与血清TGF-β1水平及CD59、DAF mRNA表达水平呈正相关(分别r=0.84,P<0.01;r=0.455,P<0.05;r=0.435,P<0.05),与BLyS mRNA表达呈负相关(r=-0.58,P<0.05);血清抗AChR-IgG抗体阳性患者中,抗AChR-IgG抗体水平与FOXP3 mRNA表达水平、血清TGF-β1水平呈负相关(分别r=-0.77、r=-0.81,均P<0.01),而与BLyS mRNA表达水平呈正相关(r=0.757,P<0.01),与CD59、DAF mRNA水平无相关性(P>0.05)。结论 FOXP3 mRNA、TGF-β1低表达可能促进B细胞活化,进而通过增加AChR抗体产生及抑制补体调节蛋白活化促进体液免疫,在MG发病中起重要作用。ABSTRACT： Objective To explore the further pathogenesis of myasthenia gravis （MG）, the correlations among the T regular cell, B cell activation and complement regulator in MG patients were analyzed. Methods The expressions of forkhead/wingedhelix transcription factor （FOXP3）, B lymphocyte stimulator （BlyS）, membrane inhibitor of reactive lysis （CD59） and decay accelerating factor （DAF） mRNA in peripheral blood mononuclear cell （PBMNCs） from 41 MG patients and 20 healthy controls were detected by RT PCR and the levels of transforming growth factor-~l （TGF-/~I） and anti-AChR antibodies in serum of MG group and Healthy control group （HC） were tested by ELISA, furthermore the relationships among them were analyzed. Results （1）The relative expression of FOXP3, CD59 and DAF mRNA in PBMNCs were significantly lower in MG group than that of healthy control group （P〈0.01） ; while expression of BLyS mRNA is increased in MG group （P〈0.01） ;meanwhile the concentration of TGF-β1 in serum were significantly lower in MG group （P〈0.01）. （2） There is no difference of the expression of FOXP3, BLyS, CD59, DAF mRNA and the serum TGF-β1 concentration between 25 ocular MG patients and 16 general MG patients （P〉0.05）; （3）From 41 MG patients, serum anti- AChR-IgG could detected in 30 patient including 18 ocular MG and 12 general MG, there were no difference of the level of anti-AChR-IgG between ocular MG subgroup and general MG subgroup; （4） The relative expression of FOXP3 mRNA in PBMNCs was positively correlated with the concentration of TGF-β1 （r= 0.84, P〈0.01） as well as the expression of CD59 mRNA （r=0. 455, P〈0.05） and DAF mRNA （r=0. 435, P〈0. 05）, but negatively correlated with the expression of BLyS mRNA （r=-0.58, P〈0.05）. What＇s more, the levels of serum anti-AChR IgG was negatively correlated with the expression of FOXP3 mRNA in PBMNCs （r= -0.77, P〈0.01） and the concentration of TGF-β1 （r= -0.81 ,P〈0.01）, but posi

关 键 词：重症肌无力 叉头样转录因子P3 转化生长因子Β1 B淋巴细胞激活因子 膜反应性溶解抑制物 衰变加速因子 

分 类 号：R746.1[医药卫生—神经病学与精神病学]