E2F-1过表达对胃癌细胞中癌基因和抑癌基因表达的影响  

INVESTIGATE DIFFERENTIALLY EXPRESSION OF ONCOGENE AND ANTI-ONCOGENE IN GASTRIC CANCER CELL LINE OVEREXPRESSION OF E2F-1

作　　者：胡锦伟[1] 肖强[1] 谢玉波[2] 李雷[1] 王长青[1] 解乃昌[1] 

机构地区：[1]广西医科大学第一附属医院胃肠腺体外科,南宁530021 [2]广西医科大学第一附属医院麻醉科

出　　处：《广西医科大学学报》2009年第3期355-358,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.30860273);广西医疗卫生重点科研课题(桂卫重200844)

摘　　要：目的:了解E2F-1过表达对胃癌细胞中癌基因和抑癌基因表达的影响,探讨E2F-1影响胃癌细胞侵袭能力的作用机制。方法:从胃癌细胞MGC-803和MGC-803/E2F-1中提取总RNA,纯化mRNA,逆转录将Cy3和Cy5两种荧光素标记到两组逆转录合成的第一链cDNA。与22K人类基因组寡核苷酸芯片进行杂交,采用LuxScan 10K/A双通道激光扫描仪扫描芯片上两种荧光信号,应用LuxScan3.0图像分析软件对图像处理分析,筛选出差异基因。结果:在21522条基因中,MGC-803/E2F-1和MGC-803细胞差异表达的癌基因和抑癌基因有39条,其中上调基因19条(49%),下调基因20条(51%)。结论:过表达E2F-1在胃癌细胞MGC-803中影响了众多癌基因和抑癌基因的表达变化,表明胃癌侵袭能力的变化是多基因相互作用,多种信号传导途径相互影响的结果。Objective： To investigate the differentially expression of oncogenes/anti-oncogenes in gastric cancer cell line ovrexpression of E2F-1 by gene chip technique, and fine the molecular mechanisms of oncogene and anti-oncogene effect on gastric cancer cell invasion capability. Methods：The total RNA was extracted from MGC-803/E2F-1 and MGC-803 gastric cancer cell line , then purified. The eDNA was obtained by reverse transcription polymerase chain reaction （RT-PCR）, and then labeled with Cy3 and Cy5 fluorescence as probes, which were hybridized with gene chip containing 22 k human genome oligonucleotide. Subsequently, the two signal images were scanned by LuxScan 10K/A dual pathways laser scanner and analyzed by LuxScan 3.0 image analysis software,then screening out differentially expressed genes. Results： Of the 215222 target genes,39 oncogenes and anti-oncogenes were screened out for differences in gene expression level between gastric cancer cell line MGC-803/E2F-1 and MGC 803. Nineteen genes were up-regulated（49%）and 20 genes were down-regulated（51%）. Conclusion ： After ovrexpression E2F-1 in vitro, the gastric cancer cell line MGC-803 effects by multiple oncogenes/anti-oncogenes expression. Subsequently,invasion capability is the effect by multiple oncogenes/anti-oncogenes and regulation in multiple signal pathways.

关 键 词：胃癌 E2F-1 基因芯片 差异表达基因 

分 类 号：R735.2[医药卫生—肿瘤]