静电纺丝素-明胶管状支架的结构与性能  

Structure and Properties of Electro-spun Silk Fibroin-Gelatin Tubular Scaffold

作　　者：王曙东[1] 张幼珠[1] 王红卫[1] 尹桂波[1] 董智慧[2] 符伟国[2] 

机构地区：[1]苏州大学纺织与服装工程学院,江苏苏州215021 [2]复旦大学附属中山医院,上海200032

出　　处：《丝绸》2009年第7期18-21,共4页Journal of Silk

基　　金：江苏省高校重点实验室开放研究课题(KJS0817)

摘　　要：为研制组织工程小口径血管,以良好生物相容性的丝素蛋白、明胶为原料,通过静电纺丝法,以高速旋转的滚轴为收集装置,构建了丝素-明胶管状支架(直径为4.5mm)。测定其形貌结构、孔隙率和溶失率,并在该支架上进行人脐静脉内皮细胞(HUVECs)培养实验。结果表明:在丝素-明胶质量比例为70∶30、纺丝液质量分数为13%、滚轴转速为1000r/min的条件下静电纺丝,可获得纤维直径较细、纤维分布较均匀、具有较高孔隙率的丝素-明胶管状支架;随着纺丝液质量分数的提高,丝素-明胶管状支架的溶失率降低,乙醇处理后管状支架溶失率大大降低;MTT显示细胞可以在支架上生长、增殖。To develop a small-diameter tissue engineering blood vessel, the biocompatible silk fibroin（SF） and gelatin were used as materials, SF-gelatin tubular scaffold with 4.5 mm diameter was fabricated via electro-spinning, which was performed on a high speed rotating mandrel. The morphology, porosity and water-dissolved rate of the SF-gelatin were measured, the HUVECs were seed on the scaffold. The results showed that the SF-gelatin tubular scaffolds could be obtained which have small diameter fibers, well-proportioned distribution of the fibers and high porosity when the proportion of SF to gelatin is 70 ： 30, mass concentration of the spinning solution is 13 %, and rotating speed is I 000 r/min. Along with the mass concentration Of the spinning solution increasing, the water-dissolved rate of the scaffold decreased, and after ethanol treating, the water-dissolved decreased greatlv. MTT showed that the HUVECs could grow and proliferate on the scaffold.

关 键 词：静电纺丝 丝素 明胶 管状支架 支架结构 孔隙率 溶失率 细胞培养 

分 类 号：TS102.512[轻工技术与工程—纺织工程]