丙酮酸乙酯干预大鼠冠状动脉微栓塞对肿瘤坏死因子α、白细胞介素1β的影响  

Effect of Ethyl Pyruvate on Tumor Necrosis Factor-α and Interferon-1β After Coronary Microembolization in Rats

作　　者：林运灵[1] 王伟伟[1] 陈宇宁[1] 李淑梅[1] 张飞龙[1] 陈良龙[1] 

机构地区：[1]福建医科大学附属协和医院心内科&福建省冠心病研究所,福建省福州市350001

出　　处：《中国动脉硬化杂志》2009年第5期371-374,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的研究丙酮酸乙酯对大鼠冠状动脉微栓塞后炎症因子表达的影响。方法通过心尖部注射大鼠自体血栓微粒建立大鼠冠状动脉微栓塞模型。36只大鼠分为假手术组、冠状动脉微栓塞未治疗组和丙酮酸乙酯预处理组,每组各12只,于术后3天处死。W estern b lot测定肿瘤坏死因子α、白细胞介素1β的表达水平,实时PCR检测肿瘤坏死因子α、白细胞介素1βmRNA表达量。结果与假手术组相比,冠状动脉微栓塞后3天,心肌组织肿瘤坏死因子α、白细胞介素1βmRNA及蛋白水平明显升高;左室功能显著恶化,左室舒张末压明显增大,左室收缩压、左室内压上升最大速率明显下降(P<0.01);心肌肿瘤坏死因子α、白细胞介素1β蛋白水平与心功能呈明显相关性。与冠状动脉微栓塞未治疗组比较,丙酮酸乙酯预处理能够显著抑制冠状动脉微栓塞后肿瘤坏死因子α、白细胞介素1β的mRNA及蛋白水平表达,改善心室功能。结论丙酮酸乙酯预处理能够抑制大鼠冠状动脉微栓塞后促炎症因子的过度激活,改善心功能。Aim To investigate the role of cytokines in myocardium following coronary microembolization （CME） and the effects of ethyl pyruvate as a myocardial protective agent in the rat CME model. Methods We created a rat model of CME by injecting a suspension of autogenic microthrombotic particles into left ventricle. 36 rats were randomly divided into 3 groups, each consisted of 12 rats ： sham-operation group （ SO）, CME model group and ethyl pyruvate intervention group （EP）. Haemodynamic ts were performed on day 3 and then the rats were sacrificed expressions of tumor necrosis factor-alpha （TNF-a） , interferon-1 （IL-1β） were analyzed by Western blotting Protein Alterations of TNF-α, IL-1β mRNA were quantitatively assessed by Real-time PCR. Results Compared with the sham group, left ventricular systolic pressure （LVSP） and the maximum rise rate of left ventricular pressure （ ＋ dp/dtmax ） significantly decreased （ P 〈 0.05 ） , while left ventricular end diastolic pressure （LVEDP） significantly increased （ P 〈 0.05 ） in the CME group. Compared with those of the sham group, the expressions of TNF-α, IL-1β protein and gene were significantly increased in CME group （ P 〈 0.05 ）. Ethyl pyruvate significantly suppressed protein and mRNA expression of TNF-α, IL-1β（ P 〈 0.05 ） , improved cardiac functions. Conclusion In this study of rat after coronary microembolization, ethyl pyruvate preserved cardiac function due to supression of the TNF-α and IL-1β.

关 键 词：冠状动脉微栓塞 缺血性心脏病 炎症因子 丙酮酸乙酯 肿瘤坏死因子Α 白细胞介素1-Β 大鼠 

分 类 号：R363[医药卫生—病理学]