整合素β1反义寡核苷酸对次声诱导ECV-304细胞F-actin表达的影响  

The effects of integrin β1 antisense oligodeoxynucleotide on the expression of F-actin in ECV-304 cells exposed to infrasound

作　　者：王冰水[1] 陈景藻[1] 郭国祯[1] 王斌[1] 袁华[1] 刘静[1] 陈丹[1] 

机构地区：[1]第四军医大学西京医院康复理疗科,西安710032

出　　处：《中华物理医学与康复杂志》2009年第6期374-376,共3页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的 研究整合素β1反义寡核苷酸（ASODN）对次声诱导人脐血管内皮细胞（ECV-304）骨架微丝F-aetin表达的影响,探讨次声信号向细胞内传导的途径.方法 应用脂质体转染试剂介导整合素β1正义寡核苷酸（SODN）和ASODN转染ECV-304,并将细胞分为次声假暴露组、次声假暴露＋ASODN组（转染整合紊β1反义寡核苷酸片断）、次声假暴露＋SODN组（转染整合素β1正义寡核苷酸片断）、次声暴露组、次声暴露＋ASODN组和次声暴露＋SODN组.次声作用频率为16 Hz,声压输出为130 dB,时间2 h.应用激光扫描共聚焦显微镜观察细胞骨架微丝聚合态肌动蛋白（F-actin）的改变,测定单个细胞F-actin的平均荧光强度.结果 次声假暴露组、次声假暴露＋ASODN组和次声假暴露＋SODN组的ECV-304细胞胞浆内有少量肌动蛋白纤维丝,方向性较差,大部分荧光物质呈弥漫状态,这3组F-actin表达差异无统计学意义.在接受次声暴露的3组细胞中,次声暴露组的F-actin表达明显增强.大多为较长的粗大应力丝,沿细胞纵轴排列较多.数量及荧光强度明显增加;与之相比,次声暴露＋ASODN组的细胞内F-actin荧光强度明显较弱;而次声暴露＋SODN组的细胞F-actin表达与次声暴露组差异无统计学意义.结论 次声作用可引起ECV-304细胞中细胞骨架F-actin表达增加,转染整合素β1 ASODN可部分抑制次声引起的F-actin表达;次声信号向细胞内传导可能与整合素-细胞骨架这一通路有关.Objective To study the effect of integrin β1 antisense oligodeoxynucleotide（ASODN）on the expression of F-actin in ECV-304 ceils exposed to infrasound,and to explore the pathway of infrasound signal transfer into the cells.Methods integrin β1 oligodeoxynucleotides were embedded in cationic liposome lipofectamine 2000 reagent and transfected into ECV-304 cells.Laser scanning confoeal microscopv was used to observe F-actin＇s expression when the cells were exposed to 16 Hz infrasound at 130 dB for 2 h.The cells＇ average fluorescence was examined using spectrofluorimetric quantification after immunofluorescent staining. Results Among the three false exposure groups,most fluorescein-labelled substances in the cells were in a diffuse state.There were few actin filaments,and they were tenuous and short, without direction.The cells＇ fluorescence intensities were almost the same.In a group exposed to infrasound,F-actin was thick and long with a longitudinal arrangement.Fluorescence intensities were strong.However,less F-actin expression was observed in the ceils of the ASODN group exposed to infrasound compared with the others similarly exposed.Correspondingly.F-actin expression in the ASODN group exposed to infrasound was almost the same as in the other exposed group.Conclusion F-actin expression can be increased in ECV-304 cells by infrasound exposure,but integrin β1 ASODN may partially inhibit its expression.The extraeellular matrix-integrin-cytoskeleton may be one of the transduetion pathways for infrasound signals into the cells.

关 键 词：整合素 寡核苷酸 次声 人脐静脉血管内皮细胞 聚合态肌动蛋白 

分 类 号：R363[医药卫生—病理学]