抑癌基因KLF6在肝癌HepG2细胞修复DNA损伤过程中的作用研究  

Effect of KLF6 gene on nucleotide excision repair capacity in human hepatocellular carcinoma cell line HepG2

作　　者：潘修成[1] 杨帆[1] 陈明[1] 郭忠胜[1] 季芳[1] 傅涓涓[1] 

机构地区：[1]徐州医学院附属医院感染性疾病科,江苏徐州221002

出　　处：《中国病理生理杂志》2009年第7期1279-1282,共4页Chinese Journal of Pathophysiology

基　　金：徐州医学院附属医院博士启动基金资助项目(No.2006B02)

摘　　要：目的:研究抑癌基因KLF6对肝癌HepG2细胞修复DNA损伤能力的影响。方法:RT-PCR及Western blotting技术测定HepG2细胞在5mg/L顺铂作用12、24、36h后KLF6 mRNA及其蛋白水平变化。采用宿主细胞再活化反应(HCR)检测KLF6稳定表达HepG2细胞修复DNA损伤的能力。结果:在5mg/L顺铂作用下,随作用时间延长,KLF6 mRNA及其蛋白水平增高,但作用36h,KLF6蛋白水平下降。KLF6稳定表达HepG2细胞修复被顺铂损伤质粒DNA的能力明显高于对照组。结论:DNA损伤能诱导肝癌HepG2细胞KLF6基因表达,KLF6基因在细胞DNA损伤修复过程中发挥一定作用。AIM ： To investigate the roles of KLF6 gene on nucleotide excision repair capacity of human hepatocellular carcinoma cell line HepG2. METHODS： Expression of KLF6 was detected by RT- PCR and Western blotting, followed by HepG2 cells treated with cisplatin （5 mg/L）. Host cell reactivation assay was used to assess nucleotide excision capacity of cisplatin - damaged HepG2 cells. RESULTS ： Upregulation of KLF6 mRNA or protein in HepG2 cells treated with 5 mg/L concentration of cisplatin was in a time - dependent manner, but KLF6 protein was degraded when exposed to cisplatin at same dose （5 mg/L） for 36 h. HCR assay showed that NER capacity of KLF6 overexpressing HepG2 cells was significantly enhanced, compared to that in control cells. CONCLUSION： DNA damage induced by cisplatin induces the expression of KLF6 gene. KLF6 gene may play an important role in mechanism of nucleotide excision repair in HepG2 cells.

关 键 词：基因 肿瘤抑制 基因 KLF6 DNA损伤 DNA修复 HEPG2细胞 

分 类 号：R735[医药卫生—肿瘤]