急性坏死性胰腺炎大鼠白细胞L-选择素表达和肌动蛋白分布的变化及中药华西胰腺炎一号的影响  

Effects of Chinese medicine WPY on expression of L-selectin and distribution of F-actin in leukocytes from acute necrotizing pancreatitis rats

作　　者：唐诗彬[1] 张肇达[2] 吴路杨[1] 廖裕彬[1] 

机构地区：[1]南方医科大学附属顺德第一人民医院普外科,广东顺德528300 [2]四川大学华西医院普外科,四川成都610041

出　　处：《中国病理生理杂志》2009年第7期1381-1385,共5页Chinese Journal of Pathophysiology

摘　　要：目的:了解胰腺局部循环内白细胞在急性坏死性胰腺炎(ANP)疾病过程中L-选择素的表达及肌动蛋白纤维多聚体(F-actin)在细胞内的分布特点,并探讨中药华西胰腺炎一号(WPY)对它们的影响。方法:44只Wistar大鼠分为正常、ANP组及ANPWPY治疗组,脾静脉采血,予CD62L单抗标记粒细胞L-选择素,TRTIC-Phalloidin标记白细胞F-actin,流式细胞仪检测粒细胞中的CD62L阳性细胞数,共聚焦激光扫描显微镜采集白细胞图像并对F-actin在白细胞内的分布进行分析。结果:ANP组脾静脉血中CD62L阳性细胞数明显高于正常对照组(11.96%±5.32%),6h(31.78%±12.37%)、12h(29.83%±13.73%)与对照组比较差异显著(P<0.05),WPY治疗组6hCD62L阳性细胞数低于ANP组。白细胞F-actin细胞边缘与中央灰度比在ANP各时段,除3h外均增高,1h(2.52±0.49)、12h(2.72±0.48)与对照组比较有显著差异(P<0.05),WPY治疗组6h(1.54±0.24)明显低于ANP组(2.35±0.55)(P<0.05)。结论:ANP胰腺局部循环内白细胞L-选择素的表达及F-ac-tin分布发生明显改变,提示白细胞黏附特性改变及变形能力下降是ANP病情进展的重要病理生理因素之一,中药华西胰腺炎一号对它们有一定的影响。AIM： To explore the changes of L - selectin expression and F - actin distribution of leukocytes from pancreatic circulation in the experimental acute necrotizing pancreatitis （ANP） rats and impact of Chinese herbs west China pancreatitis yi hao （WPY） on them in vivo. METHODS ： Forty - four Wistar rats were randomized into control group, ANP group and ANP WPY treated group. The leukocytes from blood of splenic vein were incubated with PE - anti - CD62L for L - selectin and TRTIC - Phalloidin for F - actin, then the ratio of CD62L positive granulocytes was measured by flow cytometry and leukocytes were taken images with confocal laser scanning microscope. The distribution of F - actin at the border or central part of leukocyte was analyzed by using Mias system. RESULTS ： The ratio of CD62L positive granulocytes increased in ANP rats, but those increases at 6 h （31.78% ± 12. 37% ） and 12 h （29. 83% ±13.73% ） were significant [ vs control group（ 11.96% ±5.32% ）, P 〈 0. 05 ]. The ratio of F - actin border/center gray value of leukocytes increased at 1 h, 6 h and 12 h in ANP rats, and those at 1 h （2. 52 ±0. 49） and 12 h （2.72 ±0. 48） were significant [vs control group （ 1.79 ±0. 26）, P 〈 0. 05 ]. The ratio of F - actin border/center gray value of leukocytes （ 1.54 ± 0. 24） declined significantly at 6 h in WPY - treated ANP rats （P 〈 0. 05）. CONCLUSION ： L - selectin expression and F - actin distribution change significantly in leukocytes from pancreatic circulation in ANP rats, suggesting that an increase in adhe- sive avidity and a decrease in deformability of leukocytes are pathophysiologic factors for progression of ANP, and traditional Chinese medicine WPY may regulate these changes of leukocytes in some degrees.

关 键 词：胰腺炎 急性 L-选择素 肌动蛋白 白细胞 中草药 

分 类 号：R363[医药卫生—病理学]