改进RP-HPLC法测定大鼠血浆淫羊藿苷浓度  

Determination of icariin in rat plasma in different time by improved RP-HPLC after oral administration of epimedium compound preparation

作　　者：段旭光[1] 周崇文[1] 陈科[1] 曾雷[1] 

机构地区：[1]湖南环境生物学院,湖南衡阳421005

出　　处：《湖南农业大学学报（自然科学版）》2009年第3期309-313,共5页Journal of Hunan Agricultural University(Natural Sciences)

摘　　要：用乙醚萃取大鼠血浆样品,用反相高效液相色谱法(Shim-pack VP-ODS色谱柱,等梯度洗脱)测定大鼠灌胃复方淫羊藿制剂后不同时间血浆中的淫羊藿苷质量浓度.结果表明,乙醚萃取处理后的血浆样品淫羊藿苷与复方制剂中其他组分及可能的内源性复合物能很好分离,淫羊藿苷在(0.0638～10.2000)μg/mL线性关系良好,日内精密度与日间精密度小于5%,提取回收率大于85%,样品在24h内保持稳定.To built a sensitive, specific and stable RP-HPLC combined with simple and efficient preparation for determination of icariin in rat plasma after intragastric administration with a epimedium compound preparation. Using ethylether to deal with the plasma sample, separation of icarrin, other constituents of compound preparation, and possible endogenous compounds were accomplished on Shim-pack VP-ODS chromatographic column by gradient elution .The results showed that the standard curve of icarrin was linear in the range of 0.063 8-10.200 0 μg/mL The method is accurate and precise, within-day precision and day to day precision are 〈5%, extraction recovery is 〉85%, the sample is stable in 24 h. The improved method can be used for analysis of icarrin in plasma after intragastric administration with compound recipe.

关 键 词：大鼠 淫羊藿 淫羊藿苷 反相高效液相色谱法 

分 类 号：S853.7[农业科学—临床兽医学]