SBHL对HeLa细胞c-myc,H-ras和hTRT基因表达的研究  

Sdosodine Hydrochlorida on Gene Expressions of c-myc, H-ras and hTRT of HeLa Cells

作　　者：艾金霞[1] 

机构地区：[1]北华大学医学检验学院

出　　处：《北华大学学报（自然科学版）》2009年第4期319-321,共3页Journal of Beihua University（Natural Science）

基　　金：吉林市卓依康纳中药研发基金(ZY040301)

摘　　要：目的观察澳洲茄胺盐酸盐对HeLa细胞c-myc，H—ras和hTRT基因表达的影响．方法用鼠抗人c-mycMcAb，H-ras McAb和hTRTPcAb作为一抗，生物素化羊抗鼠IgG作为二抗，用免疫组化方法测定澳洲茄胺盐酸盐对HeLa细胞c-myc，H—ms和hTRT基因的表达．结果澳洲茄胺盐酸盐处理的HeLa细胞与对照组相比，c-myc，H-ras和hTRT基因的表达均明显降低（P〈0．05）．结论澳洲茄胺盐酸盐下调HeLa细胞c-myc和H-ras基因的表达，抑制细胞增殖，诱导分化；澳洲茄胺盐酸盐下调HeLa细胞hTRT基因的表达，抑制端粒酶活性，抑制细胞生长．Objective To observe the effects of SBHL on the expressions of c-myc gene, H-ras gene and hTRT gene of HeLa cells. Methods Mouse anti-human c-myc McAb, H-ras McAb and hTRT polyclonal antibody was used specific antibody and sheep anti-mouse IgG was used the second antibody. The expressions of c-myc gene, H-ras gene and hTRT gene of HeLa cells were detected by immunohistochemistry assy. Results After treated by Sdosodine Hydrochlorida, the expressions of c-myc gene, H-ras gene and hTRT gene of HeLa cell decreased obviously（ P 〈 0.05 ）. Conclusion The expressions of c-myc gene and H-ras gene were downregulated, the growth of HeLa cell was inhibited and the differentiation of HeLa cell was induced by Sdosodine Hydrochlorida; The expressions of hTRT gene was downregulated, telomerase activity was inhibited and the growth of HeLa cell was inhibited.

关 键 词：澳洲茄胺盐酸盐 HELA细胞 C-MYC基因 H—ras基因 HTRT基因 端粒酶 

分 类 号：R733.705[医药卫生—肿瘤]