灵芝孢子粉多糖的提取及其生物活性研究  

Extraction and Biological Activity of Polysaccharide in Ganodrema Lucidiun Spore Powder

作　　者：冯翠萍[1] 王晓闻[1] 程旭东[1] 常明昌[1] 

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《中国食品学报》2009年第3期58-62,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：山西省青年科技基金研究项目(20021039);山西省科技攻关项目(021025)

摘　　要：目的:研究灵芝孢子粉多糖清除亚硝酸钠(NaNO2)和二苯代苦味酰自由基(DPPH·)的能力。方法:以提取孢子油后的孢子粉为原料,采用水提醇沉法提取多糖;以葡聚糖为标准,测定多糖含量。结果:脂肪对多糖的提取有影响,脱脂灵芝孢子粉和未脱脂灵芝孢子粉的多糖含量分别为25.53%和17.74%。在一定浓度范围内,随着多糖浓度的增加,对NaNO2和DPPH·的清除率提高。当多糖质量浓度从1.2mg/mL增加到2.5mg/mL时,对NaNO2的清除率从82.4%增加到88.3%;当多糖质量浓度从0.078mg/mL增加到5mg/mL时,对DPPH·的清除率从3.2%增加到19.9%。结论:孢子粉多糖具有清除NaNO2和DPPH·的能力。Objective： NaNO2 and DPPH. scavenging activities of polysaccharide in Ganodrema Lucidiun spore powder were investigated. Method： Polysaccharide was extracted by water and deposited by ethanol after oil extraction, using glucan as standard to analyze the content of polysaccharide. Results： The results showed that the extraction rate of polysaccharide were influenced by the exist of fat. The content of polysaccharide was 25.53% in defat Ganodrema Lucidiun spore powder, and 17.74% in non-defat Ganodrema Lucidiun spore powder. In the certain concentration, with increasing the polysaccharide concentration, NaNO2 and DPPH. scavenging rate increased. When the concentration of polysaccharide increased from 1.2 mg/mL to 2.5 mg/mL, the NaNO2 scavenging rate was increased from 82.4% to 88.3%. For the DPPH scavenging rate, the polysaccharide concentration increased from 0.078 mg/mL to 5 mg/mL, the scavenging rate increased from 3.2% to 19.9%. Conclusion： Polysaccharide in Ganodrema Lucidiun spore powder has scavenging capability of NaNO2 and DPPH.

关 键 词：灵芝孢子粉 多糖 清除NaNO2 清除DPPH· 

分 类 号：R284[医药卫生—中药学] R285.5[医药卫生—中医学]