血管新生抑制蛋白Vasostatin(120-180aa)防治兔角膜新生血管的研究  

Inhibitory effect of recombinant human vasostatin(120-180aa) on rabbit corneal neovascularization

作　　者：刘建巨[1] 张晓梅[1] 王晓丹[1] 王建文[1] 

机构地区：[1]哈尔滨医科大学附属第一医院眼科医院,黑龙江哈尔滨150001

出　　处：《中国实验诊断学》2009年第7期861-863,共3页Chinese Journal of Laboratory Diagnosis

基　　金：黑龙江省教育厅科学技术研究项目资助(11521164);哈尔滨医科大学附属第一医院科研基金资助(2007051)

摘　　要：目的探讨重组人血管抑制因子Vasostatin(120-180aa)对于碱烧伤诱导的家兔角膜新生血管的抑制作用。方法采用1 mol/L NaOH溶液烧伤家兔角膜,建立碱烧伤诱导的家兔角膜新生血管的动物模型;将家兔分为A、B、C、D4组,每组10只眼。伤后24 h后分别给予1×PBS缓冲液,20、40、80μg/mL Vasostatin(120-180aa)球结膜下注射,2次/周,共4周。测量各时间点角膜新生血管的生长面积,观察血管的生长情况。结果各时间点角膜新生血管的面积,B组、C组及D组均低于A组(P<0.05),C组及D组均低于B组(P<0.05),C组与D组相比,差异无显著性(P>0.05)。结论重组人Vasostatin(120-180 aa)蛋白可有效抑制角膜新生血管的形成。Objective To investigate the inhibitory effect of recombinant human vasostatin （ 120 - 180aa） on alkaline-induced corneal neovascularization. Methods Corneal neovascularization animal model was established using 1 mol/L NaOH solution in rabbit.The rabbit eyes were divided into 4 groups：A group （10 eyes）,B group （10 eyes）,C group （10 eyes） and D group （10 eyes）. 1 x PBS solution buffer,20,40 and 80 μg/mL vasostatin（120- 180 aa） protein was subconjunctivally injected respectively in rabbits 24 hours after burning for twice a week. The areas of rabbit corneal neovasculaxization were measured at each time point. Results The areas of corneal neovasculafization in B group, C group and D group were significantly lower than that A group at each time point （ P 〈0.05）,and those of C group and D group were lower than B group （ P 〈0.05） ,but there was not significant difference between C group and D group （ P 〉 0.05）. Conclusion The recombinant human vasostatin（ 120- 180 aa） can effectively inhibit alkaline-induced corneal neovascularization.

关 键 词：血管新生抑制蛋白 碱烧伤 角膜新生血管 

分 类 号：R772.2[医药卫生—眼科]