碳纳米管表面绿原酸印迹固相萃取材料的制备及应用  

Preparation and Application of Chlorogenic Acid Imprinted Solid Phase Extraction Materials Based on Multiwalled Carbon Nanotubes

作　　者：张华斌[1] 张朝晖[1,2] 聂燕[1] 胡宇芳[1] 邹海英[1] 姚守拙[2] 

机构地区：[1]吉首大学化学化工学院,吉首416000 [2]湖南大学化学生物传感与计量国家重点实验室,长沙410082

出　　处：《分析化学》2009年第7期955-959,共5页Chinese Journal of Analytical Chemistry

基　　金：湖南省自然科学基金(No.08JJ4004);湖南省教育厅优秀青年基金(No.07B061);湖南大学化学生物传感与计量国家重点实验室开放基金;湖南省大学生研究性创新基金资助

摘　　要：在多壁碳纳米管表面接枝的双键键合,以绿原酸为模板,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用沉淀聚合技术,在碳纳米管表面成功制备绿原酸印迹材料。采用红外光谱、扫描电镜和热重分析研究此印迹材料的性能。结果表明,在碳纳米管表面接枝一层稳定、均匀、30～40nm厚的印迹材料。采用高效液相色谱研究此印迹材料的吸附动力学及吸附容量,实验结果表明,此印迹材料对绿原酸的结合存在两个结合位点,最大吸附容量Qmax分别为21.5和32.7μmol/g。以此印迹材料作为固相萃取剂,优化萃取条件,成功应用于金银花提取液中绿原酸的富集分离研究,富集因子达25。A novel molecularly imprinted polymers （MIPs） was prepared based on muhiwalled carbon nanotubes （MWNTs） by using ehlorogenic acid as template, methacrylic acid（MAA） as the functional monomer, and ethylene glycol dimethacrylate （EGDMA） as the cross-linker. Infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis were employed to characterize the composite structure and morphology, the results showed that a stable imprinted layer was grafted on the MWNTs with the average thickness of 30-40 nm. The dynamic adsorption and static adsorption experiments were also executed and the results showed that two kinds binding sites with the maximum apparent binding constant 21. 5μmol/g and 32.7 μmol/g existed v. i. between the imprinted polymers and chlorogenic acid. As solid phase extraction （SPE） materials, the separation and enrichment of chlorogenic acid from honeysuckle were successful with a enrichment factor of 25.

关 键 词：多壁碳纳米管 分子印迹 绿原酸 分离 

分 类 号：TB383.1[一般工业技术—材料科学与工程]