团头鲂肠黏膜益生乳酸菌的分离及体外抗逆性研究  

Isolation and Resistance of Lactobacillus from Intestinal Mucosa of Bluntnose Black Bream(Megalobrama amblycephala)

作　　者：张洪波[1] 单晓枫[1] 钱爱东[1] 韩云 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]通榆县瞻榆镇畜禽防疫服务中心,吉林白城137200

出　　处：《水产科学》2009年第7期395-398,共4页Fisheries Science

摘　　要：从团头鲂肠黏膜上分离筛选出2株对嗜水气单胞菌有较强的抑制作用的乳酸菌菌株JK-1和JK-2,通过耐受胆盐、耐受pH值、耐受蛋白酶和耐高温等试验对这2株乳酸菌进行体外抗逆性试验研究。其中在胆盐耐受性试验中,JK-1和JK-2在胆盐浓度0.4%、培养4 h时仍有35%以上的存活率;在pH值耐受性试验时,2株乳酸菌均可在一定的酸性和碱性条件下存活;胰蛋白酶对2株菌的存活率没有影响;在70℃的高温中,2株乳酸菌均有一定的耐受性。Two strains of Lactobacillus JK-1 and JK-2 were isolated from intestinal mucosa of bluntnose black bream（Megalobrama amblycephala ）which showed an effective inhibition to Aeromonas hydrophila. A toleranee test was conducted for cholate, pH, protease, and high temperature. Among the eholate-tolerance test, over 35% of JK-1 and JK-2 survived after culturing for 4 and 0.4% concentration in cholate. And in pH-tolerance test, two strains of lactic acid bacteria survived in both acid and alkaline environment. Trypsin did not affect the their survival. Moreover, they performed certain tolerance to the megatemperature of 70 ℃.

关 键 词：团头鲂 乳酸菌 分离 抗逆性 

分 类 号：Q939.92[生物学—微生物学]