杉木不同杂交组合抗寒性研究  

A STUDY ON THE CHILLING RESISTANCE OF DIFFERENT CROSSES OF CHINESE FIR

作　　者：何祯祥[1,2] 施季森[1,2] 余荣卓 李梅 包宏[1] 胡兹苓[1] 

机构地区：[1]南京林业大学 [2]福建省洋口国有林场

出　　处：《南京林业大学学报（自然科学版）》1998年第2期57-59,共3页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家"八五"攻关项目;国家自然科学基金

摘　　要：对杉木４组不同杂交组合的亲本和子代用改进的ＤＥＡＥ阴离子交换柱色谱法和硅胶薄层色谱法分离制备针叶磷脂酰甘油，用气相色谱法测定了它们的脂肪酸组成。结果表明杉木针叶中ＰＧ脂肪酸的组成主要有棕榈酸、油酸、亚油酸、亚麻酸和３反式十六碳一烯酸。不同杂交组合的测定结果表明杉木抗寒性有较强的遗传性。几个测定指标中，ＩＵＦＡ（不饱和指数）较好地反映了杉木抗寒性的相对大小。The phosphatidylglycerol from the needles of different crosses of Chinese fir were isolated using our improved method of DEAE CL 6B column chromatography and silica thin layer chromatography after being extracted with chloroform and methanol. A quantitative analysis was conducted by gas chromatography on a DB FFAP column. The results showed that there were main five compositions of fatty acid in phosphatidylglycerol in the needles of Chinese fir. IUFA could reflect the ralative ability to chilling of Chinese fir, and it could be considered as one of indexes to evaluate the ability of chilling_resistance.

关 键 词：杉木 杂交 抗寒性 针叶 PG脂肪酸 

分 类 号：S791.270.4[农业科学—林木遗传育种]