苯丁酸钠和二甲基甲酰胺对乳腺癌MCF-7细胞增殖抑制及同源盒基因(HOX)A5表达影响的比较  

The effect of sodium phenylbutyrate versus that of dimethylformamide on proliferation of MCF-7 breast cancer cells and HOXA5 expression

作　　者：孙象军[1] 

机构地区：[1]山东省临沂市人民医院普外一科,276003

出　　处：《实用医学杂志》2009年第13期2025-2027,共3页The Journal of Practical Medicine

摘　　要：目的:观察苯丁酸钠(PB)和二甲基甲酰胺(DMF)对乳腺癌MCF-7细胞增殖及同源盒基因(HOX)A5表达的影响。方法:应用MTT比色法,以梯度浓度的PB或DMF作用于MCF-7细胞,分别于24、48、72h对细胞增殖进行检测。HOXA5基因表达水平用基因组与β肌动蛋白(β-actin)灰度比值表示。结果:0.391～3.125mmol/L PB和0.5～8.0mmol/L DMF作用MCF-7细胞24～72h,随着PB浓度的增加或作用时间的延长,细胞生长抑制率明显增加。随着DMF浓度的增加或作用时间的延长,细胞生长抑制率也较明显地增加。RT-PCR法检测未处理乳腺癌MCF-7细胞中HOXA5基因表达0.460±0.065。应用0.781mmol/L和1.563mmol/L PB处理后,HOXA5基因表达水平分别为0.801±0.122、0.903±0.096,HOXA5基因表达明显上调,与用药前比较,差异有统计学意义(P<0.01)。应用0.5mmol/L和1.0mmol/L DMF处理后,HOXA5基因表达水平分别为0.474±0.057、0.484±0.061,HOXA5基因表达无明显变化,与用药前比较,差异无统计学意义(P>0.05)。结论:PB和DMF可有效抑制乳腺癌MCF-7细胞的增殖,PB对HOXA5基因mRNA水平的表达有明显的上调作用,DMF对HOXA5基因表达无显著影响。Objective To investigate the effects of sodium phenylbutyrate （PB） and dimethylformamide （DMF） on proliferation of MCF-7 breast cancer cells and homeobox A5 （HOXAS） expression. Methods The proliferation of MCF-7 cells cultured with different concentrations of PB or DMF for 24 h, 48 h, and 72 h was detected by MTT assay. The expression ratio of HOXA5 gene/β-actin indicated the expression level of HOXAS. Results MCF-7 cells were treated with 0.391 - 3.125 mmol/L of PB or 0.5 - 8.0 mmol/L of DMF for 24 to 72 h. With an increase in concentration of PB or DMF or with a prolonged treatment, the inhibition of proliferation of the cancer cells was notably increased. RT-PCR revealed that HOXA5 mRNA expression was markedly upregulated after treatment with PB （[0.801 ± 0.1221 for 0.781 mmol/L and [0.903 ± 0.096] for 1.563 mmol/L vs. [0.460 ± 0.065] for pretreatment, P 〈 0.01）, while HOXA5 expression did not different significantly after treatment with DMF （ [0.474 ± 0.057] for 0.5 mmol/L and [0.484 ± 0.061 ] for 1.0 mmol/L], P 〉 0.05）. Conclusions Both PB and DMF effectively inhibit proliferation of MCF-7 breast cancer cells. PB can evidently upregulate HOXA5 mRNA expression, but DMF has no effect on the expression.

关 键 词：乳腺肿瘤 同源盒基因A5 苯丁酸钠 二甲基甲酰胺 

分 类 号：R737.9[医药卫生—肿瘤] R994.6[医药卫生—临床医学]