甲状腺肿瘤原位细胞凋亡检测与端粒酶逆转录酶表达研究  

Significance of cell apoptosis index and hTERT in thyroid tumor tissue

作　　者：黄宇[1] 梁华晟[2] 韦海明[3] 

机构地区：[1]广西壮族自治区人民医院肝胆腺体外科,广西南宁530021 [2]广西医科大学第九附属医院内分泌科,广西北海536000 [3]广西壮族自治区人民医院病理科,广西南宁530021

出　　处：《中国现代医学杂志》2009年第12期1876-1878,共3页China Journal of Modern Medicine

基　　金：广西青年科技自然基金课题(No:桂科表0728108);广西卫生厅自筹经费科研基金课题(No:Z2007003)

摘　　要：目的研究原位细胞凋亡指数(apoptosis index,AI)及端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)在原发性甲状腺肿瘤中表达及其与病变的关系。方法对87例原发性甲状腺肿瘤(乳头状腺癌38例,滤泡状腺癌21例,未分化癌5例,腺瘤23例)分别应用原位细胞凋亡检测(TuNEL)试剂盒测定AI及应用免疫组织化学方法检测hTERT表达情况。结果AI及hTERT在腺癌表达显著高于腺瘤组(P<0.01);AI及hTERT灰度值呈负相关性(r=-0.38,P<0.05)。结论AI及hTERT的表达与甲状腺肿瘤显著相关,检测AI及hTERT水平对鉴别甲状腺肿瘤性质具有重要参考意义。[Objective] To study the relationship between AI, hTERT and thyroid tumor tissue by combining assays of AI and hTERT in thyroid tumor tissue. [Methods] AI was evaluated by TUMEL method and the expression of hTERT were dectected by immunohistochemistry in 87 cases （thyroid papillary cancer 38, thyroid follicular cancer 21, thyroid undifferentiated cancer 5, thyroid adenoma 40）. [Results] The expression of AI and hTERT in thyroid carcinoma increased （P 〈0.01）. There has negative relationship between AI and hTERT （r =-0.38, P 〈0.05）. [Conclusion] It is suggested that combined detections of AI and hTERT may have important reference significance on the differentiation of thyroid benign or malignant tissue.

关 键 词：甲状腺癌 细胞凋亡 端粒酶逆转录酶 鉴别诊断 

分 类 号：R736.1[医药卫生—肿瘤] R392.11[医药卫生—临床医学]