大蜡螟抗菌物质的抑菌活性检测及其初步分离  

Detection to Antimicrobial Activity and Prefractionation of Antimildew Agent from Calleria mellonella

作　　者：赵晴[1] 李静[1] 陆秀君[1] 李瑞军[1] 董建臻[1] 谢咸升[1,2] 

机构地区：[1]河北农业大学植物保护学院,河北保定071001 [2]山西省农科院小麦研究所,山西临汾041000

出　　处：《中国农学通报》2009年第13期166-170,共5页Chinese Agricultural Science Bulletin

基　　金：河北省自然科学基金项目"高活性昆虫抗菌肽的筛选及其对植物致病菌抑菌活性研究"(C200700450)

摘　　要：以大蜡螟末龄幼虫为材料,诱导后获得免疫血淋巴为抗菌粗提物,并分为新鲜,冷冻,加热3种不同的处理,进行了对2种常见细菌和16种植物病原真菌的抑菌活性检测,并进行了Sephadex G-50凝胶过滤分离。结果表明,大蜡螟抗菌物质对大肠杆菌没有明显的抑制作用,而对金黄葡萄球菌抑制作用明显,抑菌活性单位在10左右;对10种植物病原真菌均有不同的抑菌活性,其中对苹果树皮腐烂病菌的抑制率达到65%以上;经过凝胶过滤分离,及对分离组分的抑菌活性测定,筛选出较合适的洗脱液为50mM乙酸铵,各分离组分抑菌活性差异明显,粗提物被分成了两个差异显著的部分。In this study, the antimicrobial crude extract was obtained as immunizing hemolymph from the last stage larvae of Calleria mellonella after induction. With three disparate treatments, fresh, frozen and heated, the crudes was carried out detection to two common bacteria and 16 kinds of plant pathogenic fungi, and separation with Sephadex G-50 gel filtration. The results showed that the three disparate treated substances have no visible antibacterial effect to E. coli, but a obvious effect to Staphylococcus aureus with inhibition of about 10 bacteriostasis units; have dissimilar effects to 10 kinds of fungi among the total kinds, and have a good inhibitory effect to Cytospora.coccodes （Wallr.）Hughes with fungistasis rate above 65%. After the gel filtration and activity detection, 50 mM ammonium acetate was screened out as the more suitable eluents, the crudes were separated into strikingly unlike parts with obviously different antimicrobial activity.

关 键 词：大蜡螟 抗菌物质 抑菌活性检测 凝胶过滤 

分 类 号：S476[农业科学—农业昆虫与害虫防治]