短发夹RNA抑制三氧化二砷耐药的白血病K562／AS2细胞MRP1基因表达的实验研究  

Inhibitionai effect of small hairpin RNA on expression of MRP1 gene in K562/AS2 cell line resistant to As2O3

作　　者：苏晓星[1,2] 卓家才[2] 古庆利[2] 熊文杰[2] 陶小梅[3] 李明[3] 张琼丽[3] 黄瑞宏[3] 刘焕勋[2] 蔡力生[2] 杜新[3] 

机构地区：[1]广州医学院研究生院,510182 [2]深圳市第二人民医院血液科 [3]深圳市第二人民医院血液病研究所

出　　处：《白血病．淋巴瘤》2009年第7期388-391,共4页Journal of Leukemia & Lymphoma

摘　　要：目的研究短发夹RNA（shRNA）对耐三氧化二砷（As2O3）的白血病K562／AS2细胞MRP1基因表达的抑制作用。方法设计并合成3条针对MRP1基因的shRNA序列，在脂质体的介导下转染K562／AS2细胞；用荧光实时定量PCR分析MRP1 mRNA的表达水平；流式细胞术检测MRP1蛋白表达和细胞内柔红霉素蓄积量。结果经MRP1 shRNA作用24h后，K562／AS2细胞株中MRP1 mRNA和蛋白表达水平明显下降，最大下调幅度分别为（79．1±0．07）％和（62．48±0．86）％（P〈0．05），同时细胞内柔红霉素蓄积量显著增加（P〈0．05）。结论MRP1 shRNA可抑制As2O3，耐药的白血病细胞株K562／AS2细胞MRP1基因的表达。Objective To investigate the inhibitional effect of MRPI-shRNA on expression of MRPI gene in K562/AS2 cells resistant to arsenic trioxide. Methods Three pieces of MRPI-shRNA were designed, synthesized and transfected into K562/AS2 cells with liposome. Expression level of MRP1 mRNA were determined by real time fluorescent quantitative PCR. MRP1 protein expression and intracellular accumulation of DNR were assayed with flow cytometry. Results After treated with MRPI-shRNA, the expression level of MRP1 mRNA and MRP1 protein in K562/AS2 cells decreased significantly（79.1±0.07） % and （62.48±0.86） %, respectively （P 〈0.05）. The intracellular accumulation of DNR increased significantly（P 〈 0.05）. Conclusion MRPI-shRNA can down-regulate the expression of MRP1 gene in K562/AS2 cell line.

关 键 词：RNA 砷剂 抗药性 肿瘤 基因 MRP1 K562细胞 

分 类 号：R733.7[医药卫生—肿瘤]