异柠檬酸裂解酶抑制剂对L-谷氨酸合成的影响  

Influences of Isocitrate Lyase Inhibitors on the Synthesis of L-Glutamate

作　　者：余秉琦[1] 蔡志强[1] 王利群[1] 杨林松[1] 朱劼[1] 朱孝霖[1] 李亮[1] 

机构地区：[1]江苏工业学院化学化工学院生物与制药工程系,江苏常州213164

出　　处：《安徽农业科学》2009年第23期10844-10845,10870,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究琥珀酸和苹果酸对L-谷氨酸合成的影响。[方法]以谷氨酸棒杆菌的典型菌株Corynebacterium glutamicumATCC13032为供试菌株,研究琥珀酸和苹果酸对细胞生长、L-谷氨酸的合成、发酵液中的残糖量以及细胞中异柠檬酸裂解酶活性的影响。[结果]琥珀酸对异柠檬酸裂解酶的活性具有部分抑制作用。在0～5.0 g/L范围内,琥珀酸的添加提高了L-谷氨酸发酵的糖酸转化率,细胞生长和L-谷氨酸分泌受到抑制,残糖量增大。苹果酸的添加降低了异柠檬酸裂解酶的活性和L-谷氨酸的产量,残糖量和细胞生长没有明显的规律性变化。同时添加琥珀酸和苹果酸各2.0 g/L时,异柠檬酸裂解酶的活性和细胞生长都有所下降,L-谷氨酸的产量和残糖量没有明显变化。[结论]添加琥珀酸增加了L-谷氨酸的产量,添加苹果酸则降低了L-谷氨酸的产量。[ Objective ] The purpose was to research the influences of succinic acid and malic acid on the synthesis of L-glutamate. [ Method ] With the typical strain Corynebacterium glutamicum ATCC 13032 of Corynebacterium glutamate as tested strain, the influences of succinic acid and malic acid on cell growth, synthesis of L-glutamate, residual sugar content in fermentation broth and activity of isocitrate lyase in cell were researched. [ Result] The succinic acid had partial inhibition on the activity of isocitrate lyase. In the range of 0 -5.0 g/L, the addition of succinic acid enhanced the glucose acid invert ratio of L-glutamate fermentation, the cell growth and secretion of L-glutamate were inhibited and the residual sugar content was increased. The addition of malic acid decreased the activity of isocitrate lyase and the output of L-glutamate and the residual sugar content and cell growth had no obvious regular change. When 2.0 g/L succinic acid and 2.0 g/L malic acid were added simultaneously, both the activity of isocitrate lyase and cell growth got decreased a little and the output of L-glutamate and residual sugar con- tent had no obvious change. [ Conchision ] The addition of succinic acid increased the output of L-glutamate and the addition of malie acid decreased the output of L-glutamate.

关 键 词：谷氨酸棒杆菌 异柠檬酸裂解酶 L-谷氨酸 

分 类 号：O641.4[理学—物理化学]