塞来昔布抑制Tca8113细胞释放PGE_2及抑制细胞增殖的作用  

Effect of celecoxib on inhibition of PGE_2 released by Tca8113 cells and proliferation

作　　者：李伟忠[1] 王晓燕[2] 丁彦青[2] 

机构地区：[1]南方医科大学南方医院口腔科,广州510515 [2]南方医科大学南方医院病理科,广州510515

出　　处：《现代口腔医学杂志》2009年第4期419-422,共4页Journal of Modern Stomatology

基　　金：广东省自然科学基金资助(06024396)

摘　　要：目的研究选择性环氧化酶2抑制剂塞来昔布对人舌鳞癌Tca8113细胞生长抑制、诱导细胞凋亡与释放前列腺素E2(PGE2)的影响。方法采用四唑氮蓝(MTT)法检测细胞增殖,AnnexinV-FITC/PI双标记法检测细胞凋亡,酶联免疫吸附实验(ELISA)检测PGE2含量,AO/EB荧光双染观察凋亡细胞的形态学变化。结果塞来昔布以剂量依赖方式抑制Tca8113细胞生长及释放PGE2,其抑制PGE2释放与抑制细胞生长及诱导细胞凋亡均呈负相关,AO/EB荧光双染观察发现经塞来昔布处理24h后,细胞出现典型的凋亡变化,凋亡细胞多见。结论塞来昔布抑制人舌鳞癌Tca8113细胞生长及诱导细胞凋亡与其抑制PGE2释放密切相关。Objective To study the effect of selective COX - 2 inhibitor celecoxib on growth inhibition, the apoptosis induction and the release of prostaglandin E2 in human tongue squamous carcinoma cell line Tca8113. Methods The anti - proliferative effect of celecoxib was measured by MTT assay. The prostaglandin E2 （ PGE2 ） levels in the supernatant of cultured Tca8113 cells were detected with enzyme- linked immunoabsordent assay （ELISA）. Annexin V- FITC/PI double labeling method was employed to detect the cell apoptosis. The morphological changes were observed by fluorescence microscopy. Results Tca8113 cell grouth and PGE2 released were inhibited by celecoxib in a dose - dependent manner. Its correlation between the release of PGE2 and both cell growth inhibition and apoptosis was negative. AO/EB fluorescence double staining showed that apoptotic cells were found AO - and EB - positive in celecoxib treated groups. Conclusion The studies conformed that there was a close relationship between the release inhibition of PGE2 and apoptosis induction and growth inhibition in Tca8113.

关 键 词：舌肿瘤 癌 鳞状细胞 环氧化酶-2 塞来昔布 前列腺素E 

分 类 号：R739.8[医药卫生—肿瘤]