TACE联合抗血管生成疗法治疗兔VX2肝移植瘤的实验研究  

Empirical Study on the Effect of Transcatheter Arterial Chemoembolization Combining Endostatin on the Rabbit VX2 Hepatic Implantation Tumor

作　　者：蹇兆成[1] 孙业全[1] 王滨[1] 刘峰[1] 刘艳[1] 白旭明[1] 宁厚法[1] 

机构地区：[1]潍坊医学院附属医院影像中心,261031

出　　处：《临床放射学杂志》2009年第7期1013-1017,共5页Journal of Clinical Radiology

基　　金：山东省科技发展计划项目(2007GG30002032);山东省卫生系统杰出学科带头人基金项目(2006-39);山东省教育厅重点项目(J04E01);潍坊医学院青年教师基金项目(KQ07021)

摘　　要：目的观察经导管动脉化疗栓塞(TACE)联合应用血管生成抑制剂内皮抑素(endostatin,ES)对兔VX2肝移植瘤疗效的影响。材料与方法30只兔VX2肝移植瘤模型随机分为对照组、TACE组(碘油+阿霉素)和抗血管生成组(碘油+阿霉素+ES),每组10只。种植后3周行TACE术,术后1周获得病理标本,分别利用免疫组织化学法和原位末端标记法检测所有标本的肿瘤微血管密度(microvascular density,MVD)及残瘤细胞的增殖、凋亡情况,并分析增殖指数(proliferation index,PI)和凋亡指数(apoptosis index,AI);术前、术后行MSCT和超声检查并计算肿瘤增长率(growth rate,GR)。结果三组肿瘤GR(%)分别为270.86±148.94、-8.91±21.77和-20.40±36.07,治疗后TACE组和抗血管生成组肿瘤体积均减小,与对照组比较差异性有统计学意义(P<0.01),各组MVD值分别为80.00±17.14、84.22±16.45、57.00±13.26,抗血管生成组最低,与其余两组比较差异均具有统计学意义(P<0.05),对照组和TACE组两组间差异无统计学意义(P>0.05);各组PI值(%)和AI值(%)分别为52.84±7.37、84.53±5.31、79.98±8.38和3.58±0.066、12.28±0.85、14.39±1.32;PI值对照组最低,与其余两组比较差异有统计学意义(P<0.01),抗血管生成组低于TACE组,但无统计学意义(P>0.05);AI值抗血管生成组最高,各组两两比较差异均具有统计学意义(P<0.01)。结论兔VX2肝移植瘤介入治疗中应用ES可直接减少肿瘤微血管的生成,间接影响肿瘤细胞的增殖和凋亡,两种疗法均可抑制肿瘤的生长,联合治疗效果更好。Objective To investigate the effect of transcatheter arterial chemoembolization （TACE） combining endostatin in rabbit VX2 hepatic implantation tumor. Materials and Methods Thirty rabbits hepatic implantation tumor model were randomly divided into three groups including control group, TACE group and anti-angiogenenic group, with ten rabbits in each. The TACE were operated three weeks after the implantation. For control group, normal saline was administered via the hepatic artery. For TACE group, lipiodol （0.2ml/kg） and ADM （2mg/kg） were administered. Lipiodol （0.2ml/kg）, ADM （2mg/kg） and endostatin （2mg/kg） were administered for each rabbit in anti-angiogenenic group. Seven days after operation, the rabbits were sacrificed and the tumors were taken out. Immunohistochemistry and TUNEL were performed to demonstrate the expression of MVD as well as the proliferation and apoptosis of residual tumor cells to analysis proliferation index （PI） and apoptosis index （AI）. MSCT and ultrasound were performed to observe the growth of the tumor before and after the TACE. Results The growth rates（%） were 270.86 ±148.94, -8.91 ±21.77 and -20.40 ±36.07 in 3 groups respectively. The tumor size in control group increased obviously, and there was significant difference of growth rate between control group and the other two groups （P 〈 0.01 ）. There was no significant difference between TACE group and anti-angiogenenic group （P 〉0.05）. The value of MVD were 80.00 ± 17.14, 84.22± 16.45 and 57.00 ± 13.26 in 3 groups respectively. Among the three groups, the MVD of anti-angiogenenic group was minimum. There was significant difference of MVD between control group and TACE group （ P 〈 0.05 ）, and no significant difference existed between control group and TACE group. The value of PI（ % ） and AI（ % ） were 52.84 ± 7.37, 84.53 ± 5.31,79.98 ± 8.38 and 3.58 ± 0. 066, 12.28 ± 0.85, 14.39 ± 1.32 in 3 groups respectively. The PI value of the control group was the lowest, whi

关 键 词：肝肿瘤 化学栓塞 血管生成抑制剂 内皮抑素 

分 类 号：R735.7[医药卫生—肿瘤]