高脂饲料诱导小鼠脂肪肝模型的建立  

High Fatty Liver Model in Mice Established by High-fat Feed

作　　者：刘锋[1] 许继凡[1] 罗婧[1] 石小凤[1] 王苏燕[1] 向志钢[2] 

机构地区：[1]吉首大学医学院06级临床医学系,湖南吉首416000 [2]吉首大学医学研究所,湖南吉首416000

出　　处：《中国民族民间医药》2009年第13期67-69,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：吉首大学校级科研项目(08JDX027);首大学大学生研究性学习和创新性实验计划项目(教通【2008】22)

摘　　要：目的:利用高脂饲料建立小鼠脂肪肝模型。方法:40只昆明小鼠,随机均分为两组,分别给予高脂饲料和普通饲料连续喂养6周,比较两组血清和肝脏生化、肝组织病理学指标的差异。结果:6周后,与对照组相比,模型组肝指数((5.9±0.19)%)、血清甘油三脂(TG,1.90±0.55mmol/L)、总胆固醇(TC,4.55±0.23mmol/L)、谷丙转氨酶(ALT,170.34±9.32U/L)、谷草转氨酶(AST,158.86±18.45U/L)肝匀浆丙二醛(MDA,5.88±1.07U/mgpro)显著升高(P<0.05)、肝匀浆超氧化物歧化酶(SOD,68.87±7.89U/mgpro)、谷胱甘肽过氧化物酶(GSH-PX,45.41±9.87U/mgpro)明显降低(P<0.05),镜下亦可见弥漫性肝细胞脂肪变性,大量炎性细胞浸润。结论:通过六周的高脂饲料诱导,成功建立小鼠脂肪肝模型。Objective To establish a fatty liver model in mice by high-fat feeding. Method 40 Kunming mice were divided into 2 groups randomly.One of them, model group, was fed with a high-fat feed; the other, control group, was fed with normal diet.After six weeks, all the mice were killed and the serum TG, TC, ALT, AST and hepatic TG, TC, MDA, SOD, GSH-PX were detected, as well as hepatic histological changes.Result Compared with the control group, the liver index （（5.9±0.19） %） , the serum TG （1.90±0.55 mmol/L） , TC （4.55±0.23 mmol/L） , ALT （170.34±9.32 U/L） , AST （158.86±18.45 U/L） , hepatic MDA （5.88± 1.07 U/mg pro） of the model group were markedly higher （P〈0.05） , the hepatic SOD （68.87±7.89 U/mg pro） and the hepatic GSH-PX （45.41±9.87 U/mg pro） were significantly lower （P〈0.05） . The histopathology research showed hepatoeellular diffuse steatosis in the model group, with a mass of inflammatory cell infiltration. Conclusion The fatty liver model in mice were established successfully for 6 weeks fed with high-fat feed.

关 键 词：脂肪肝 小鼠 高脂饮食 动物模型 

分 类 号：R589.2[医药卫生—内分泌]