猪粪便不同酸糖化预处理及其酶解条件研究  

Study on Different Acid Pretreatment of Swine Manure and Enzymatic Hydrolysis

作　　者：桑宇[1] 李静[2,3] 凌娟[2] 晏飞来[2] 徐静静[2] 何咏[2] 刘兴礼[2] 张莲姬[2] 

机构地区：[1]重庆医科大学药学院,重庆400016 [2]西南大学资源环境学院,重庆400716 [3]重庆市农业资源与环境研究重点实验室,重庆400716

出　　处：《西南师范大学学报（自然科学版）》2009年第4期102-105,共4页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：重庆市北碚区科委科技计划项目(2007-13);重庆市科委自然科学基金计划资助项目(CSTC;2006BB7335);西南大学本科生科技创新基金项目

摘　　要：探讨了几种酸对猪粪便糖化预处理和预处理后的猪粪便酶解条件的影响.结果表明:强酸预处理猪粪便优于中强酸和弱酸,而5%盐酸在110℃预处理猪粪便4 h可以使还原糖含量达到46.41%.盐酸预处理猪粪便后,影响其酶解的主要因素是酶用量和底物浓度,酶解试验的最佳条件为酶解温度45℃、酶用量300 mg/L、底物浓度15 g/L和pH 4.8.酶解还原糖含量达到11.26%.This paper discussed the effect of swine manure pretreatment by different acid and studied the optima for enzymatic hydrolysis of swine manure after HCl pretreatment. The result showed that the strong acid pretreatment for swine manure were better than these by medium strong acid and weak acid pretreatment, and the optical pretreatment condition for swine manure was concentration of HCl 5 %, temperature 45℃ and treatment 4h to get 46.41% yield of reduced sugar. After pretreated HCl, factors affecting the enzymatic hydrolysis were enzyme loading and substrate concentration. The favorable conditions for enzymatic hydrolysis were determined to be temperature 45 ℃, enzyme loading 300 mg· L^-1 sub strate, substrate concentration 15 g· L^-1 and pH 4.8.

关 键 词：酸预处理 猪粪便 糖化 酶解 

分 类 号：Q814.9[生物学—生物工程]