早期乳腺癌hMAM mRNA阳性循环肿瘤细胞检测及其临床意义  

Detection and Clinical Significance of hMAM mRNA-positive Circulating Tumor Cells in Early Breast Cancer

作　　者：陈艳[1] 伍治平[1] 王熙才[1] 金从国[1] 刘馨[1] 周永春[1] 谷玉兰[1] 邹天宁[2] 

机构地区：[1]昆明医学院第三附属医院肿瘤研究所,650118 [2]昆明医学院第三附属医院乳腺科,650118

出　　处：《肿瘤防治研究》2009年第7期578-582,共5页Cancer Research on Prevention and Treatment

基　　金：云南省教育厅基金资助项目(04Y029C)

摘　　要：目的研究早期乳腺癌hMAM mRNA阳性循环肿瘤细胞检测的临床意义。方法巢式RT-PCR检测50例早期乳腺癌患者术后辅助治疗前外周血hMAM mRNA阳性细胞,随访。24例乳腺良性疾病患者和20例健康体检志愿者作对照。结果早期乳腺癌患者术后辅助治疗前外周血有核细胞hMAM mRNA阳性率26.0%,与良性乳腺疾病患者(4.2%)、健康体检志愿者(0%)比较,差异有统计学意义(分别为P=0.025、P=0.012);其hMAM mRNA阳性率与癌组织HER2过表达相关(P=0.037);13例阳性患者中8例(61.5%)随访出现复发转移(P=0.004),中位无瘤生存期明显降低(P=0.002)。结论hMAM mRNA是检测乳腺癌循环肿瘤细胞较为理想的分子标记物。早期乳腺癌患者术后辅助治疗前hMAM mRNA阳性循环肿瘤细胞检测,可能是预测复发转移和预后不良的辅助指标。Objective To study clinical significance of the presence of hMAM mRNA-positive circulating tumor cells in early breast cancer. Methods Peripheral blood was obtained from 50 early breast cancer patients after surgical removal of the primary tumor and before the initiation of adjuvant therapy and analyzed for the presence of hMAM mRNA-positive ceils using a nested RT-PCR methodology. All patients were followed up. 20 healthy individuals and 24 patients with breast benign lesions served as controls. Results hMAM mRNA-positive cells were detected in 26.0% of early breast cancer patients, 4. 2%of breast benign lesions patients, but not in healthy individuals （P = 0. 025, P = 0. 012 respectively）. A higher incidence of hMAM mRNA-positive cell detection was associated with overexpression of HER2 receptor on the primary tumor （P = 0. 037）. 8/13 （61.5%） of early breast patients with hMAM mRNA positive cells relapsed during the follow-up period （P = 0. 004）. Detection of peripheral-blood hMAM mRNA-positive cells was associated with reduced median relapse-free interval（P = 0. 002）. Conclusion hMAM mRNA is a specific and suitable molecular marker for the detection of circulating tumor ceils in early breast cancer. Detection of peripheral blood hMAM mRNA-positive circulating tumor cells might have an important predictive and prognostic value as a marker of relapse and poor clinical outcome in early breast cancer patients.

关 键 词：乳腺肿瘤 循环肿瘤细胞 微转移 HMAM 

分 类 号：R737.9[医药卫生—肿瘤]