2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖诱导人结肠癌SWlll6凋亡的作用  

Experimental study on apoptosis of human colon carcinoma cell line SWlll6 induced by 2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-Glucose

作　　者：寇炜[1] 吴静[2] 赵晋[1] 兰咏梅[1] 窦春江[1] 王爱勤[3] 

机构地区：[1]西北民族大学医学院技能教研室,甘肃兰州730030 [2]北京大学第九临床医院消化科,北京100038 [3]中国科学院兰州化学物理研究所,甘肃兰州730000

出　　处：《第四军医大学学报》2009年第14期1289-1291,共3页Journal of the Fourth Military Medical University

基　　金：西北民族大学中青年科研项目(D2004-021)

摘　　要：目的:研究D-葡萄糖衍生物2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖(COPADG)对人结肠癌SWlll6细胞生长增殖的影响,进而探讨COPADG诱导SWlll6细胞凋亡的作用.方法:用不同浓度的COPADG处理SWlll6,采用MTT法测定其对SWlll6细胞生长增殖的抑制作用,通过DNA琼脂糖凝胶电泳、流式细胞术观察其对SWlll6细胞诱导凋亡的效应.结果:COPADG能抑制SWlll6细胞的生长增殖,并呈一定的浓度、时间依赖性;COPADG能诱导结肠癌SWlll6细胞凋亡;DNA琼脂糖凝胶电泳呈现典型的DNA梯形条带图谱;流式细胞仪检测出现典型的亚二倍体凋亡峰.结论:COPADG在体外可显著诱导结肠癌SWlll6细胞凋亡.AIM ： To investigate the effect of 2-[（3-carboxy-1-oxoprogy1） amino] -2-deoxy-D-Glucose（COPADG ） on human colon carcinoma cell line SW1116 and to study the function of the COPADG in inducing cell line SW1116 apoptosis. METHODS-SW1116 cells were treated with the COPADG at various concen-trations. MIT assay was used to examine the growth and proliferation of SW1116 cells after treatment with the COPADG. Agarose gel electrophoresis of DNA fragmentation and flow cytometry were used to analyze the cell line SW1116 apoptosis. RESULTS： COPADG significantly inhibited the growth and proliferation of cell line SW1116 in a dose-and time-dependent manner. Agarose gel electrophoresis analysis revealed DNA ladder and atypical sub- diploid peak was observed by flow cytometry. CONCLUSION： COPADG may inhibit SW1116 cell growth and proliferation through inducing apoptosis.

关 键 词：2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖 细胞凋亡 SW1116细胞 

分 类 号：R735.7[医药卫生—肿瘤]