大肠癌组织CD137L的表达及意义  

Expression of CD137L in Colorectal Cancer Tissues and Its Significance

作　　者：杨琳[1,2] 黄娜[1] 雷尚通[3] 李爱民[1] 吕成伟[1] 廖旺军[1] 罗荣城[1] 

机构地区：[1]南方医科大学南方医院肿瘤中心,广东广州510515 [2]解放军广州疗养院一科 [3]南方医科大学南方医院普外科,广东广州510515

出　　处：《中山大学学报（医学科学版）》2009年第4期381-385,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金(2007005208)

摘　　要：【目的】检测大肠癌组织及癌旁组织中CD137LmRNA及蛋白的表达情况,探讨其在大肠癌发生进展中的意义。【方法】运用实时荧光定量逆转录聚合酶链反应及冰冻切片免疫组化的方法检测大肠癌组织及癌旁组织中CD137L的表达情况,并分析其表达情况与各临床因素的相关性。【结果】54例大肠癌组织中CD137LmRNA的相对表达水平为0.035±0.013,显著高于癌旁组织的0.008±0.005(P<0.05)。但在不同性别、年龄、肿瘤发生部位、Dukes分期、病理分化及血清CEA水平情况下,CD137LmRNA的表达水平无显著性差异(P>0.05)。随机抽取8例大肠癌肿瘤组织及3例癌旁组织,进行免疫组化检测,结果显示6例CD137L蛋白呈阳性表达,CD137L表达于肿瘤细胞表面;3例癌旁组织该分子的表达均为阴性。【结论】CD137LmRNA在大肠癌组织中高表达,CD137L分子表达于大肠癌肿瘤细胞表面,提示该分子可能在大肠癌的发生进展中发挥作用。[Objective] To detect the expression of CDI37L mRNA and protein in colorectal cancer tissues and discuss its possible clinical significance. [Methods] To detect the expression of CD137L mRNA in colorectal cancer tissues and adjacent noncancerous tissues by real-time RT-PCR and immunohistochemistry and to analyze the relationship between CD137L expression and clinical features. [Results] The mean level of CD137L mRNA in colorectal cancer tissues is significantly higher than that of adjacent noncancerous tissues （0.035 ± 0.013 vs. 0.008 ± 0.005, P 〈 0.05 ）. CD137L mRNA expression in colorectal cancer show no significant differences among different age, sex, tumor location, Dukes classification, histological differentiation, and serum levels of CEA. We selected 8 colorectal cancer tissues and 3 adjacent tissues randomly to analyze the expression of CD137L by immunohistochemistry. CD137L was expressed on tumor cells in colorectal cancer tissues. The expression of CD137L was observed only in colorectal cancer tissues （6/8）, but not in adjacent noncancerous tissues （0/3）. [Conclusions] The expression of CD137L Mma in colorectal cancer tissues is higher than that of adjacent noncancerous tissues and CD137 is expressed on tumor cell surface, it might be of biological importance for the progression of colorectal cancer.

关 键 词：CD137L CD137 聚合酶链反应 免疫组化 结直肠癌 

分 类 号：R735.35[医药卫生—肿瘤]