Analysis of RNA-Dependent RNA Polymerase Sequence of Infectious Flacherie Virus Isolated in China and Its Expression in BmN Cells  

作　　者：LI Ming-qian LU Qi-neng WU Xiao-Feng MAN Na-na CHEN Xiao-xue JIN Wei LU Xing-meng 

机构地区：[1]Laboratory of lnvertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, P.R.China

出　　处：《Agricultural Sciences in China》2009年第7期872-879,共8页中国农业科学（英文版）

基　　金：supported by the National 863 Program of China (2006AA10A119);the Key Project of Science and Technology Commission of Zhejiang Province,China (2003C22013)

摘　　要：Full gene sequence of RNA-dependent RNA polymerase （RdRp） from Bombyx mori infectious flacherie virus isolated in Zhejiang Province, China （Zhejiang01/CHN/2002） was cloned. The sequence was 1 920 nucleotides in length coding 639 amino acid residues. Sequences comparison of RdRp showed Zhejiang01/CHN/2002 was 99.7% nucleotide sequence and 99.1% amino acids sequence homology with Japanese strain. The RdRp sequence was aligned with 8 representative picorna（-like） viruses and 8 highly conserved regions were detected. The result indicated their relevance function. Phylogenetic tree of 14 picorna（-like） viruses which RdRp presumed protein sequences revealed that the viruses from Iflavirus genus formed an independent clade. The RdRp was successfully expressed in BmN cells using Bac-to-Bac expression system.Full gene sequence of RNA-dependent RNA polymerase （RdRp） from Bombyx mori infectious flacherie virus isolated in Zhejiang Province, China （Zhejiang01/CHN/2002） was cloned. The sequence was 1 920 nucleotides in length coding 639 amino acid residues. Sequences comparison of RdRp showed Zhejiang01/CHN/2002 was 99.7% nucleotide sequence and 99.1% amino acids sequence homology with Japanese strain. The RdRp sequence was aligned with 8 representative picorna（-like） viruses and 8 highly conserved regions were detected. The result indicated their relevance function. Phylogenetic tree of 14 picorna（-like） viruses which RdRp presumed protein sequences revealed that the viruses from Iflavirus genus formed an independent clade. The RdRp was successfully expressed in BmN cells using Bac-to-Bac expression system.

关 键 词：infectious flacherie virus RNA-dependent RNA polymerase phylogenetic analysis BmN cells EXPRESSION 

分 类 号：S763.12[农业科学—森林保护学]