抗癌活性肽对胆囊癌GBC-SD细胞作用前后的基因表达谱研究  

Analysis of gene expression pattern in gallbladder carcinoma cells treated by anti-cancer bioactive peptide (ACBP)

作　　者：云强 苏秀兰[2] 欧阳晓晖[2] 

机构地区：[1]内蒙古医院,100027 [2]内蒙古医学院一附院

出　　处：《中国实用医药》2009年第5期10-12,共3页China Practical Medicine

摘　　要：目的研究抗癌活性肽对人胆囊癌细胞系GBC-SD细胞作用前后的基因表达谱,筛选与抗癌活性肽作用相关的基因表达改变。方法将1152条人类全长基因的PCR产物按微矩阵排列点样于特殊处理的玻片上制备成表达谱芯片;按一步法抽提抗癌活性肽作用前后体外培养的胆囊癌细胞的RNA并纯化mRNA,通过逆转录用两种荧光Cy3-dUTP和Cy5-dUTP标记对照和实验组胆囊癌细胞cDNA,制成cDNA探针并与表达谱芯片杂交,用ScanArray4000扫描仪扫描芯片上两种荧光信号,计算机分析判定差异表达的基因。结果在1152条基因中筛选出有差异表达的基因245条,其中上调的121条,下调的124条。结论利用本基因表达谱芯片可同时研究数以千计的基因表达水平,从而在基因水平上探讨抗癌活性肽对胆囊癌GBC-SD细胞作用的机制。Objective To investigate gene expression pattern of human gallbladder carcinoma cell line treated by anti-cancer bioactive peptide （ ACBP）, and in the identification of novel cance rassociated genes. Methods The cDNA retro-transcribed from equal quantity mRNA from the gallbladder carcinoma cell line （GBC-SD） which were labeled with Cy5 and Cy3 fluorescence as a probes. The mixed probe was hybridized with cDNA microarray which representing a set of 1152 human genes. It was scanned by laser scanner. The acquired image was analyzed by software. Results 245 differentially expressed genes were identified that further identified 121 upregulated and 124 downregulated gene. Conclusion cDNA microarray for analysis of gene expression patterns is a powerful method to identify nove cancer as sociated genes.

关 键 词：抗癌活性肽 胆囊癌 细胞系 基因芯片 基因表达谱 

分 类 号：R735.8[医药卫生—肿瘤]