灰玫瑰青霉产生的抗菌物质AF1的分离与活性研究  

Isolation and characterization of antibacterial factor AF1 produced by Penicillium griseoroseum

作　　者：杨炜华[1,2] 卢雪梅[1] 张为灿[1] 高培基[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,山东济南250100 [2]山东省医学科学院基础医学研究所,山东省现代医药与技术重点实验室,山东济南250062

出　　处：《山东大学学报（理学版）》2009年第7期1-4,共4页Journal of Shandong University(Natural Science)

基　　金：国家自然科学基金资助项目(30600026);山东省优秀中青年科学家科研奖励基金资助项目(2007BS03002)

摘　　要：从灰玫瑰青霉Penicillium griseoroseum发酵液中提取到1种具有抗菌活性的物质AF1,对其性质进行初步研究。发酵液经纱布过滤,离心后经5ku超滤膜超滤,经Sephadex LH20和HPLC分离纯化得到纯净物质AF1,并对其生物学活性进行研究。结果证明:AF1对β-内酰胺酶和酸碱稳定,对热有较好的耐受性,不是青霉素类抗菌物质;相对分子质量约为1465,结构中含有糖和氨基特征,对金黄色葡萄球菌的MIC为80μg/mL,对抗药金黄色葡萄球菌有效且不容易诱导细菌抗药性产生。An antimierobial factor termed AF1 was isolated from culture broth of Penicillium griseoraseum. The filtered fermentation broth underwent eentrifugation and ultrafihration （membrane cutoff 5 kD）. Then it was purified using Sephadex LH-20 and high-perfomumce liquid chromatography （HPLC）. The molecular weight of AF1 was 1465 Da. AF1 was stable to β-laetamase, hydroxylamine, acid, alkali and heat. Together, these data indicate that AF1 is a novel antibacterial factor which is different from penicillin. The molecular structure of AF1 shows characteristics of saceharide and amid. MIC of AF1 versus Staphylococcus aureus is 80 μg/ml, and AF1 is effective to drug-resistant S. aureus. It is difficult to induce bacterial resistant to AF1.

关 键 词：青霉 抗菌 分离纯化 生物活性 

分 类 号：Q939.9[生物学—微生物学]