活化转录调控因子2(ATF-2)在下颌骨髁突软骨细胞中调控bcl-2机制的研究  

Role of activating transcription factor 2 in the growth of mandible condyle chondrocytes

作　　者：马秦[1] 宋红[2] 程晓兵[1] 张浚睿[1] 陈富林[3] 吴炜[1] 毛天球[1] 

机构地区：[1]西安第四军医大学口腔医院颌面外科,710032 [2]西安第四军医大学口腔医院综合科,710032 [3]西安西北大学生命科学学院组织工程实验室

出　　处：《实用口腔医学杂志》2009年第4期459-462,共4页Journal of Practical Stomatology

基　　金：陕西省科技厅社发攻关资助基金〔编号:2007K14-06(4)〕

摘　　要：目的:研究ATF-2通过bcl-2影响髁突软骨生长发育的机制。方法:应用体外培养原代小鼠髁突软骨细胞,原代细胞基因转染,蛋白印迹杂交等技术,阐明ATF-2调控bcl-2表达的机制。结果:外源性的ATF-2在髁突软骨细胞中,与bcl-2的调控子中CRE结合,增强bcl-2调控子的活性;当CRE位点的序列改变,ATF-2不能在髁突软骨细胞中对bcl-2调控子的活性产生影响。结论:ATF-2在软骨细胞中,通过影响有凋亡抑制作用的bcl-2的活性和表达,参与下颌骨髁突软骨的生长发育。Objective： To study the role of activating transcription factor 2 （ATF-2） in the growth of mandibular condyle cartilage. Methods： Primary chondrocytes of condyle were cultured. Expression plasmid of ATF-2 and plasmid bcl-2 promoter were transfected into chondroeytes. Luciferase assay and Western blot were used. Results： The absence of ATF-2 in mandibular condyle chondrocytes resulted in a decline in bcl-2 promoter activity, reduction in bcl-2 protein level. Conclusion： The results strongly imply that ATF-2 is required for adequate bel-2 expression, and play a significant role in controlling growth plate ehondrocyte progression.

关 键 词：ATF-2 BCL-2 下颌骨髁突 软骨细胞 

分 类 号：R78[医药卫生—口腔医学]