口腔鳞癌组织与口腔正常黏膜组织蛋白质差异表达的初步研究  

A primary study of the different protein expression profiles between human oral squamous cell carcinoma and normal oral mucosa tissues

作　　者：王文梅[1] 郑春兰[1] 胡勤刚[1] 蒋文晖[1] 黄晓峰[1] 唐巍[1] 

机构地区：[1]南京大学医学院附属口腔医院,210008

出　　处：《实用口腔医学杂志》2009年第4期540-543,共4页Journal of Practical Stomatology

基　　金：2007年南京市医学科技发展重大项目

摘　　要：目的:筛选口腔鳞癌及正常口腔黏膜组织的差异表达蛋白质,为研究口腔鳞癌发生机制提供实验依据。方法:收集10例口腔鳞癌组织及正常口腔黏膜组织,进行二维电泳,选择在表达差异量较大的29个点进行质谱和生物信息学分析,确定所分析的蛋白质类型。结果:口腔鳞癌及相应正常口腔黏膜组织凝胶的平均蛋白质点数分别为2325±390和2487±281。双向凝胶电泳图显示,口腔鳞癌及正常口腔黏膜组织的差异表达蛋白质点数为29个,这29个点在癌组织中均为低表达,对其进行了质谱(PMF)和生物信息学分析,鉴定了其中的3个点,它们是:β纤维蛋白(fibrin beta)、磷酸丙糖异构酶(tri-osephosphate isomerase TIM)、unknown蛋白。结论:β纤维蛋白、磷酸丙糖异构酶、unknown蛋白在口腔鳞癌发生发展过程中发生了改变,其机制尚待进一步阐明。Objective： To identify the different protein expression profiles between human oral squamous cell carcinoma （OSCC） and normal oral mucosa tissues, and provide experimental data for further study of the development mechanism of OSCC. Methods： 10 cases of OSCC and paired normal oral mncosa tissues were collected and analyzed through two-dimensional gel electrophoresis （2- DE） and matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS）. Results： （1） The average protein spots of OSCC were 2 325 ± 390, while that of normal oral mucosa tissues were 2 487 ± 281. （2） 29 differential protein spots were found between OSCC and normal oral mucosa. Moreover, these protein spots were all down-regulated in OSCC compared with normal oral mucosa. Among these spots, 3 were identified as fibrin beta, triosephosphate isomerase （TIM） and unknown protein through mass spectrometry and bioinformation. Conclusion： Down- regulation of fibrin beta, Triosephosphate isomerase （TIM） and unknown protein are found in the development of OSCC and the mechanism needs further study.

关 键 词：口腔 鳞癌 双向凝胶电泳 蛋白质组 肽质量指纹图谱 

分 类 号：R739.8[医药卫生—肿瘤] R781.5[医药卫生—临床医学]