Emdogain作用下人牙周膜细胞的差异表达基因  

Different gene expressions in human periodontal cells after being treated with Emdogain

作　　者：张雪洋[1] 赵华[1] 章锦才[1] 

机构地区：[1]广东省口腔医院,510280

出　　处：《实用口腔医学杂志》2009年第4期556-559,共4页Journal of Practical Stomatology

基　　金：广东省科技计划项目(编号:2006B35801010);广东省医学科研基金资助项目(编号:A2006113)

摘　　要：目的:筛选猪釉基质蛋白(EMD,Emdogain)作用于人牙周膜细胞后的差异表达基因。方法:培养第4代牙周膜细胞,实验组加入终浓度为100μg/mlEMD的无血清DMEM培养液,对照组为不含EMD的无血清DMEM培养液,培养3d,采用基因芯片技术检测相关的差异表达基因。结果:共得到112条差异表达基因,上调基因101条,下调基因11条。结论:EMD作用于人牙周膜细胞后,与细胞的生长、增殖,细胞外基质的合成,新生血管的形成,骨形成等功能相关的基因表达都显著提高,而脂蛋白代谢相关基因和角化细胞生长相关基因的表达下调。Objective：To study the different gene expressions in human periodontal ceils after being treated with Emdogain（EMD）. Methods： The fourth passage periodontal cells were cultured. Cells in experimental group were cultured with 100 μg/ml EMD in non- serum DMEM for 3 days. Cells in control group were cultured in non-serum DMEM for 3 days. The gene expressions in human perio- dontal cells were screened with gene chip technique. Results.. There were 112 differently expressed genes, 11 down-regulated genes and 101 up-regulated genes. Conclusion： After treated with Emdogain, expression of genes related to cell growth, cell proliferation, biosynthesis of extraeellular matrix, angiogenesis and osteogenesis is up-regulated dramatically. Genes related to lipid metabolization and keratinocyte growth is down-regulated in human periodontal cells.

关 键 词：釉基质蛋白 牙周膜细胞 差异表达基因 

分 类 号：R78[医药卫生—口腔医学]