血管紧张素转换酶抑制剂联合奈达铂对鼻咽癌CNE2细胞的抑制作用  

Inhibitory Effect of Angiotensin-converting Enzyme Inhibitor Combined with Nedaplatin on Human Nasopharyngeal Carcinoma CNE2 Cells

作　　者：莫正英[1] 李志玖[1] 梁清乐[2] 

机构地区：[1]郧阳医学院附属太和医院肿瘤科,湖北十堰442000 [2]郧阳医学院附属生命科学研究所,湖北十堰442000

出　　处：《郧阳医学院学报》2009年第3期233-236,共4页Journal of Yunyang Medical College

摘　　要：目的:研究血管紧张素转化酶抑制剂(ACE-I)联合奈达铂(NDP)在体外对鼻咽癌细胞生长的影响。方法:利用MTT比色法、流式细胞仪、RT-PCR、免疫印迹及酶联免疫等方法测定不同组(对照组、NDP组、ACE-I组、NDP+ACE-I组)对人鼻咽癌细胞CNE2生长、细胞周期及对血管内皮生长因子(VEGF)表达的影响。结果:单独NDP或ACE-I不能抑制CNE2细胞生长,联合应用能明显抑制CNE2细胞生长和VEGF的表达。结论:ACE-I和NDP联合抑制CNE2细胞增殖是通过抑制VEGF的表达实现的。Objective To investigate the effect of angiotensin-converting enzyme inhibitor combined with nedaplatin on the growth of nasopharyngeal carcinoma CNE2 cells in vitro. Methods The effects of ACEI combined with nedaplatin on cell proliferation,cell cycle and VEGF expression of CNE2 were detected with MTT assay, flow cytometry, RT-PCR, Western blotting and ELISA, respectively. Results Neither NDP nor ACE-I had significant inhibitory effect on the CNE2 growth, while the combined application had significant inhibitory effect on the CNE2 growth VEGF expression. Conclusion The proliferation of CNE2 could be inhibited with the combination of ACE-I and NDP by inhibiting VEGF expression.

关 键 词：血管紧张素转化酶抑制剂 奈达铂:VEGF CNE2 

分 类 号：R739.63[医药卫生—肿瘤]