Characterization of clarithromycin resistance in Malaysian isolates of Helicobacter pylori  

作　　者：Norazah Ahmad Wan Rasinah Zakaria Sheikh Anwar Abdullah Ramelah Mohamed 

机构地区：[1]Bacteriology Unit,Institute for Medical Research [2]Department of Internal Medicine,Universiti Kebangsaan Malaysia Medical Centre [3]Universiti Kebangsaan Malaysia Molecular Biological Institute,Universiti Kebangsaan Malaysia Medical Centre

出　　处：《World Journal of Gastroenterology》2009年第25期3161-3165,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by A grant from the Ministry of Science,Technology and Innovation,Malaysia

摘　　要：AIM： To characterize the types of mutations present in the 23S rRNA genes of Malaysian isolates of clarithromycin-resistant Helicobacter pylori （H pylorl~. METHODS： Clarithromycin susceptibility of H pylori isolates was determined by E test. Analyses for point mutations in the domain V of 23S rRNA genes in clarithromycin-resistant and -sensitive strains were performed by sequence analysis of amplified polymerase chain reaction products. Restriction fragment length polymorphism was performed using Bsa I and MboI enzymes to detect restriction sites that correspond to the mutations in the clarithromycin- resistant strains. RESULTS： Of 187 isolates from 120 patients, four were resistant to clarithromycin, while 183 were sensitive. The MIC of the resistant strains ranged from 1.5 to 24 pg/mL. Two isolates had an A2142G mutation and another two had A2143G mutations. A T2182C mutation was detected in two out of four clarithromycin-resistant isolates and in 13 of 14 clarithromycin-sensitive isolates. Restriction enzyme analyses with Bsa I and Mbo I were able to detect the mutations. CONCLUSION： Clarithromycin resistance is an uncommon occurrence among Malaysian isolates of Hpylori strains and the mutations A2142G and A2143G detected were associated with low-level resistance.AIM:To characterize the types of mutations present in the 23S rRNA genes of Malaysian isolates of clarithromycin-resistant Helicobacter pylori (H pylori).METHODS: Clarithromycin susceptibility of H pylori isolates was determined by E test. Analyses for point mutations in the domain V of 23S rRNA genes in clarithromycin-resistant and-sensitive strains were performed by sequence analysis of amplified polymerase chain reaction products. Restriction fragment length polymorphism was performed using Bsa-RESULTS: Of 187 isolates from 120 patients, four were resistant to clarithromycin, while 183 were sensitive. The MIC of the resistant strains ranged from 1.5 to 24 μg/mL. Two isolates had an A2142G mutation and another two had A2143G mutations. A T2182C mutation was detected in two out of four clarithromycin-resistant isolates and in 13 of 14 clarithromycin-sensitive isolates. Restriction enzyme analyses with Bsa-CONCLUSION: Clarithromycin resistance is an un-common occurrence among Malaysian isolates of H pylori strains and the mutations A2142G and A2143G detected were associated with low-level resistance.

关 键 词：Clarithromycin resistance Helicobacter pylori 23S rRNA mutation Restriction fragment length polymorphism 

分 类 号：R573[医药卫生—消化系统]