人工养殖长鳍篮子鱼消化道指数及3种消化酶活性分布  

The digestive tube index and the activity distribution of three kinds of digestive enzymes in the digestive organs of cultured Siganus canaliculatus

作　　者：杨金海[1,2] 章龙珍[1,2] 庄平[1,2] 赵峰[1] 刘鉴毅[1] 张涛[1] 侯俊利[1] 姚静梅[2] 

机构地区：[1]中国水产科学研究院东海水产研究所,农业部海洋与河口渔业重点开放实验室,上海200090 [2]上海海洋大学水产与生命学院,上海200090

出　　处：《海洋科学》2009年第7期43-50,共8页Marine Sciences

基　　金：国家科技支撑计划项目(2006BAD03B08);国家863计划项目(2004AA603110);国家科技基础条件平台项目(2006DKA30470-004);上海市高校水产养殖学E-研究院建设项目(E03009)

摘　　要：以体质量58.51g±21.15g、体长13.08cm±1.856cm的长鳍篮子鱼(Siganus canaliculatus)为实验材料,对其消化道指数和主要消化酶活性分布进行了研究。采用常规方法测定了长鳍篮子鱼的消化道指数,其比内脏重、比肝重、比胃重、比幽门盲囊重、比肠重、比肠长分别为0.1572±0.0230、0.0166±0.0060、0.0115±0.0070、0.0043±0.0021、0.0202±0.0102、2.595±0.457;体质量与体长的回归方程为Y=0.0806X2.5457(r=0.9777,P<0.01)。蛋白酶在各消化器官中的比活力顺序为幽门盲囊>肠>肝脏>胃;淀粉酶的比活力顺序为肠>幽门盲囊>胃>肝脏;脂肪酶比活力顺序为幽门盲囊>肝脏>肠>胃;肠的总蛋白酶活、总淀粉酶活、总脂肪酶活在各消化器官中都最高。肝脏、胃、肠、幽门盲囊的A/P值分别为2.15、22.65、3.81、1.96。研究表明,肠道是长鳍篮子鱼消化食物的最重要的消化器官;根据消化道指数、消化酶分布、A/P值,表明长鳍篮子鱼是偏植食性为主的杂食性鱼类。The digestive tube index and the activity distributions of three kinds of digestive enzymes in the digestive organs of cultured Siganus canaliculatus （average body weight of 58.51 g± 21.15 g and body length of 13.08 cm±1.856 cm） were studied in the paper. With a normal method, the digestive tube indices of S.canaliculatus were measured. The ratio of intestine gut length to body length was 2.595±0.457 while the ratios of viscera, liver, stomach, pyloric caecum and intestine weight to body weight were 0.157 2±0.023 0, 0.016 6±0.006 0, 0.011 5±0.007 0, 0.004 3±0.002 1 and 0.020 2±0.010 2, respectively. The exponential regression equation of body weight and body length was Y=0.080 6X^2.5457 （r=0.977 7, P〈0.01 ） . The specific activity ofprotease in different organs was in the order of pyloric caecum〉 intestine〉 liver 〉 stomach, while amylase in the order of intestine〉 pyloric caecum〉 stomach〉 liver, and lipase in the order of pyloric caecum〉liver〉intestine〉stomach. The total protease activity, amylase activity and lipase activity in intestine were much higher than that in other digestive organs, respectively. The A/P values of liver, stomach, intestine, pyloric caecum were 2.15, 22.65, 3.81, 1.96 respectively. The result showed that the intestine was the most important functional organ for digestion and S. canaliculatus was a kind of polyphagia fish that leaned to be herbivorous based on the digestive tube indices, the activity distributions of three kinds of digestive enzymes and A/P values.

关 键 词：长鳍篮子鱼(Siganus canaliculatus) 消化道指数 蛋白酶 淀粉酶 脂肪酶 酶活 

分 类 号：S917[农业科学—水产科学]