番红褪色法检测羟自由基清除效果研究  

The Method of Detecting Hydroxyl Radical Scavenged by Faded Safranine

作　　者：祁红兵[1] 陆萍 陈钧[3] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000 [2]励才实验学校,江苏姜堰225300 [3]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《信阳师范学院学报（自然科学版）》2009年第3期448-450,共3页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：信阳师院青年骨干教师资助计划

摘　　要：根据Fenton反应原理,利用番红作为显色剂检测由EDTANa2-Fe(Ⅱ)-H2O2体系产生的.OH.番红褪色光度法测定清除羟自由基的最佳反应条件为:往10 mL具塞试管中,依次加入pH7.4的磷酸缓冲液1.0mL,番红溶液(520 mg/L)0.2 mL,EDTANa2-Fe(Ⅱ)(0.003 8 mol/L)1.0 mL,不同浓度的样品溶液0.1mL,最后加入0.25%H2O20.5 mL,双蒸水稀释至刻度后于40℃保温30 min,紫外-可见分光光度法519 nm处测吸光度A.Based on the priciple of Fenton reaction, the ·OH produced by the EDTANa2- Fe （Ⅱ ）-H2O2 system can be detected by using safranine as developer. The optimal experimental conditions is evaluated： 1.0 mL pH7.4 phosphate buffer; safranine（520 mg/L） 0.2 mL, EDTANa2-Fe （ Ⅱ ） （0.003 8 mol/L） 1.0 mL,and 0.25% H2O2 0. 5 mL. All these agent must be added one by one into the 10 mL capped test tube. Redistilled water was diluted to graduation line, then incubated at 40 ℃. The amount of hydroxyl radical produced in the system can be indirectly assayed through absorbance change at a wavelength of 519 nm（△A519 ） by means of UV-Vis spectrophotometer.

关 键 词：羟自由基 清除作用 分光光度法 

分 类 号：Q935[生物学—微生物学]