HPLC法测定血府逐瘀汤中阿魏酸的含量  

HPLC Determination of Ferulic Acid in Xuefu Zhuyu Decoction

作　　者：叶会呈[1] 叶其馨[1] 王带媚[1] 曾丽云[1] 

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《中药新药与临床药理》2009年第4期356-358,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立血府逐瘀汤中阿魏酸含量的测定方法。方法采用高效液相色谱法,色谱柱:Kromasil C18(250mm×4.6mm,5μm);流动相:乙腈-0.085%磷酸(17∶83);流速:1.0mL/min;检测波长:316nm;柱温:35℃。结果阿魏酸在0.0252μg～0.504μg范围内线性关系良好,r=0.9999,平均回收率为100.15%,RSD=1.69%。结论该法简单,快捷,适用于血府逐瘀汤中阿魏酸的含量测定。Objective To establish a HPLC method for the determination of ferulic acid in Xuefu Zhuyu Decoction. Methods A Kromasil C18（250 mm × 4.6 mm, 5μm） column was adopted. The mobile phase consisted of acetonitrile -0. 085 % phosphoric acid （17 ： 83）with the flow rate of 1.0 mL/min. The column temperature was at 35 ℃ and detection wavelength was set at 316 nm. Results A good linearity of ferulic acid was in the range of 0. 0252 μg to 0. 504 μg and r = 0. 999 9. The average recovery was 100. 15 % and RSD = 1.69 %. Conclusion The method is simple and rapid, and it is suitable for the determination of ferulic acid in Xuefu Zhuyu Decoction.

关 键 词：血府逐瘀汤 阿魏酸 含量测定 HPLC 

分 类 号：R284.1[医药卫生—中药学]