乙酸灌肠加束缚应激致大鼠肠易激综合征模型的建立及其评价  

Establishment and evaluation of a rat IBS model induced by intracolonic instillation of acetic acid and restraint stress

作　　者：安钰[1] 白殿卿[2] 付健[3] 孙晓宁[4] 

机构地区：[1]宁夏医科大学,宁夏回族自治区银川市750004 [2]海南省人民医院实验动物中心,海南省海口市570311 [3]海南医学院药物安全评价中心,海南省海口市571101 [4]海南省人民医院消化内科,海南省海口市570311

出　　处：《世界华人消化杂志》2009年第15期1548-1551,共4页World Chinese Journal of Digestology

基　　金：海南省自然科学基金资助项目;No.30855~~

摘　　要：目的:探讨腹泻型肠易激综合征(D-IBS)动物模型的建立方法.方法:♂Wistar大鼠40只,随机分为IBS1组、IBS2组、灌肠对照组及正常对照组,每组10只,采用乙酸灌肠加束缚应激和Williams方法制造IBS动物模型,用腹壁撤退反射(AWR)评分和腹外斜肌放电活动检测对其内脏敏感性,同时行组织学检查对肠黏膜的组织学改变进行评价.结果:两模型组大鼠AWR评分和腹外斜肌收缩次数在不同扩张容量(1.0、1.5、2.0mL)下均较对照组明显增加(F=6.318,3.978,7.810,均P<0.05;F=6.318,3.978,7.810,均P<0.05).组织学分析显示各组大鼠均无明显的炎症性表现.结论:乙酸灌肠加束缚应激和Williams方法制成的IBS动物模型均符合IBS的内脏敏感性机制,可用于IBS的实验研究.AIM： To explore the method to establish a rat model of diarrhea-predominant irritable bowel syndrome and to provide experimental evidence for future research. METHODS： Forty male Wistar rats were divided into four groups： IBS1 group, IBS2 group, positive group and normal control group. A rat model of IBS was established by intracolonic instillation of acetic acid and restraint stress and using Williams method. Visceral hypersensitivity was evaluated by abdominal with-drawal reflex and abdominal electrical activity, while histological diversification was evaluated by histological examination. RESULTS： Visceral hypersensitivity （abdominal with-drawal reflex and abdominal electrical activity） was significantly enhanced in two model groups than two control groups （F = 6.318, 3.978, 7.810, all P 〈 0.05; F =6.318, 3.978, 7.810, all P 〈 0.05）. There were no signs of obvious inflammation in the rats of each group. CONCLUSION： A rat model of IBS induced by intracolonic instillation of acetic acid and restraint stress and Williams method is in accord with visceral hypersensitivity of IBS, feasible for experimental study.

关 键 词：腹泻型肠易激综合征 大鼠模型 腹壁退反射 腹外斜肌放电活动 

分 类 号：R574[医药卫生—消化系统] R-332[医药卫生—内科学]