正丁酸盐诱导的大鼠非炎症性结肠致敏模型  

A butyrate-induced model of non-inflammatory colonic hypersensitivity in rats

作　　者：叶建明[1] 胡品津[2] 薛存宽[3] 何学斌[3] 蒋朋[3] 汪晓珍[4] 

机构地区：[1]中山市人民医院消化内科,广东省中山市528403 [2]中山大学附属第一医院消化内科,广东省广州市510080 [3]华中科技大学同济医学院老年医学研究所,湖北省武汉市430077 [4]中山市人民医院儿科,广东省中山市528403

出　　处：《世界华人消化杂志》2009年第15期1552-1557,共6页World Chinese Journal of Digestology

基　　金：广东省中医药局建设中医药强省科研基金资助项目;No.2007290~~

摘　　要：目的:研究正丁酸盐结肠灌注对大鼠结肠敏感性的影响,探讨结肠致敏模型制作的新方法.方法:Wistar大鼠每天接受浓度为300mmol/L丁酸盐1mL从肛门灌肠2次,共6d.分别在造模3、6d后以半定量的方法观察大鼠对直肠扩张(CRD)的反应,进行结肠敏感性评估,记录初始感觉压力和最大耐受压力.实验后处死大鼠,取距肛门10cm的结直肠,观察肠黏膜损伤指数,病理检查观察组织病理改变.结果:模型组和盐水对照组均未见黏膜及黏膜下层组织结构破坏,黏膜下嗜酸性粒细胞计数、肥大细胞计数无显著差异.造模6d模型组结肠运动最大压力较盐水对照组明显升高,差异有显著意义(t=9.25,P<0.01).模型组初始压力感觉及最大耐受压力较盐水对照组明显降低,差异有显著意义(t=36.71,15.02,均P<0.01).结论:正丁酸盐灌肠可以使结肠敏感性增加,不破坏结肠黏膜组织结构,结肠黏膜致敏与肥大细胞无关.AIM： To investigate Butyrate-enema＇s efficacy on visceral hypersensitivity in rats and to explore a new method of establishing a model of noninflammatory colonic hypersensitivity in rats. METHODS： Rats of the model group received enemas of 1 mL of butyrate solution （300 mmol/L） twice a day for 6 d. After 3 d or 6 d when the model of visceral hypersensitivity was built, scores of abdominal withdrawal reflex （AWR） in colorectal distention （CRD） were recorded. Hypersensitivity parameters were determined thresholds of initial Meanwhile, the pressure sensation and most endurance thresholds were recorded. Macroscopic and histologic activities of MPO and counting of mast leukocyte and eosinophile granulocyte were performed on colonic mucosa tissue at the end of the experiment. RESULTS： Macroscopic and histologic activities of MPO analyses, counting of mast leukocyte and eosinophile granulocyte were not significantly different between control group and experimental group. At day 6, there was a significant increase in scores of CRD in experimental group against control group for the most pressure （t = 9.25, P 〈 0.01）. There were a significant increase in average and highest pressure of colorectal motion in experimental group against control group （t = 36.71, 15.02, both P 〈 0.01）. CONCLUSION： Butyrate-enema may lead to colonic hypersensitivity while not bring damages to colonic mucosal structure. Colonic hypersen sitivity has no correlation with mast cells.

关 键 词：正丁酸盐 肠易激综合征 内脏 高敏性 大鼠 直肠扩张 

分 类 号：R574.62[医药卫生—消化系统] R-332[医药卫生—内科学]