结肠黏膜低度炎症对大鼠内脏感觉的影响  

Dextran sulfate sodium-induced low grade mucosal inflammation activates visceral hypersensitivity in rats

作　　者：王承党[1] 郑雪雁[1] 郑玮玮[1] 

机构地区：[1]福建医科大学附属第一医院消化内科福建医科大学消化系病研究室,福建省福州市350005

出　　处：《世界华人消化杂志》2009年第16期1621-1625,共5页World Chinese Journal of Digestology

基　　金：福建医科大学第一临床医学院学科带头人基金资助项目;No.JXK200701~~

摘　　要：目的:探讨结肠黏膜低度炎症(low grade mucosal inflammation,LGMI)对大鼠内脏感觉的影响.方法:健康♂SD大鼠40只,随机分为LGMI组和对照组(n=20).LGMI组大鼠给予15g/L葡聚糖硫酸钠(DSS)饮用7d,然后饮用蒸馏水7d,对照组大鼠饮用蒸馏水.第14天进行气囊扩张大鼠腹部回缩反射(AWR)评分和腹壁肌电测定;实验结束后取结肠组织作常规病理学检查;免疫组织化学染色观察腰膨大部脊髓c-Fos、P物质(SP)和降钙素基因相关肽(CGRP)表达变化.结果:LGMI组大鼠结肠黏膜炎症评分为1.56±0.78分,对照组为0.46±0.54分,2组比较差异有显著性意义(P=0.003).当结肠气囊压力为20mmHg时,2组大鼠的AWR评分和腹壁肌电幅值差异均无统计学意义;当气囊压力为40、60、80mmHg时,LGMI组的AWR评分和腹壁肌电幅值明显高于对照组(均P<0.05).LGMI组大鼠腰骶段脊髓c-Fos、SP、CGRP的平均吸光度值均高于对照组(165.26±10.12 vs 126.52±11.48,134.28±10.62 vs 120.82±8.92,157.66±6.25 vs 118.67±5.68,均P<0.01).结论:LGMI触发了内脏感觉过敏,可能在IBS的发病中起重要作用.AIM： To investigate the role of low grade mucosal inflammation （LGMI） induced by dextran sulfate sodium （DSS） in visceral sensitivity of the rats. METHODS： Forty healthy male Sprague-Dawley （SD） rats were randomly divided into LGMI group and control group （n = 20）. LGMI was induced by addition of 15 g/L DSS to drinking water for 7 d, and then distilled water for another 7 d. The control group rats were given distilled water alone. At the 14th day, abdominal withdrawal reflex （AWR） and myoelectric amplitude （MA） of the oblique externus abdominis muscle were recorded respectively after colonic distention byballoon with ascending internal pressure from 20 mmHg to 80 mmHg. The colonic tissue samples were taken for histopathologic examinations （HE staining）, and the expressions of c-fos, substance P （SP） and calcitonin gene-related peptide （CGRP） in the intumescentia lumbalis were detected using immunohistochemistry. RESULTS： The colonic mucosal inflammation score in LGMI rats was 1.56±0.78, significantly higher than that in control rats （0.46± 0.54, P = 0.003）. Neither the AWR score nor the MA had significant difference between the two groups when the internal balloon pressure was 20 mmHg. When the pressure was up to 40, 60, 80 mmHg, the AWR score and the MA in LGMI rats were significantly higher than those in the control group （all P 〈 0.05）. The expression of c-fos, SP and CGRP in LGMI were respectively higher than those in controls （165.26 ±0.12 vs 126.52 ±11.48, 134.28 ± 10.62 vs 120.82 ± 8.92, 157.66 ± 6.25 vs 118.67± 5.68, all P 〈 0.01）. CONCLUSION： Low grade inflammation of colonic mucous induced by DSS in rats may promote expression of the c-Fos, SP, and CGRP in the intumescentia lumbalis, and activate the visceral hypersensitivity, which underlies IBS.

关 键 词：肠易激综合征 黏膜低度炎症 内脏感觉过敏 腹壁退缩反射 腹壁肌电 葡聚糖硫酸钠 

分 类 号：R574[医药卫生—消化系统]