西安地区幽门螺杆菌cagA,iceA基因与其所致疾病的相关性  

Association of cagA and iceA genotypes with Helicobacter pylori related diseases in Xi'an area

作　　者：张翔[1] 阎志[1] 曲凯[1] 徐纪茹[1] 韩燕[2] 乔文[3] 陈艳炯[1] 

机构地区：[1]西安交通大学医学院免疫学与病原生物学系,陕西省西安市710061 [2]西安交通大学医学院遗传与分子生物学系,陕西省西安市710061 [3]西安交通大学医学院第一附属医院消化内科,陕西省西安市710061

出　　处：《世界华人消化杂志》2009年第16期1632-1637,共6页World Chinese Journal of Digestology

基　　金：国家大学生创新性实验基金资助项目;No.610737~~

摘　　要：目的:研究幽门螺杆菌(Hpylori)cagA、iceA基因及其不同组合对Hpylori感染结局的影响,探讨西安地区Hpylori的优势致病基因型.方法:用快速尿素酶试验(rapid urease test,RUT)筛选出Hpylori阳性胃黏膜标本101例,细菌基因组DNA提取试剂盒提取DNA,用聚合酶链反应(PCR)扩增尿素酶C(ureC)基因的方法筛选出Hpylori阳性标本91例.经PCR及琼脂糖凝胶电泳对cagA,iceA的基因亚型进行检测,用χ2检验以及Fisher精确检验分析各基因及其不同组合与疾病的相关性.结果:cagA基因的阳性率为79.1%,iceA的总检出率为75.82%,其中iceA1为50.5%,iceA2为38.5%,cagA+/iceA1+的检出率高于其他组,单一基因及其不同组合在各疾病组中分布没有显著差异.iceA与cagA存在相关性.iceA2分别发现有229、334、439、549bp以及229bp+334bp的基因片段.结论:cagA+/iceA1+是西安地区Hpylori的优势致病基因型,cagA、iceA1、iceA2各单一基因以及其不同组合与感染的临床结局无关.iceA与cagA基因可能存在协同作用,该地区iceA2基因有较大的变异性.AIM： To study the impact of cagA, iceA genes and their synergy on the outcome of H pylori infection so as to explore the dominant genotypes of H pylori that induce certain diseases in Xi＇an area. METHODS： One hundred and one H pylori-positive specimens from the patients after gastroscopy were preliminarily selected using the rapid urease test （RUT） and 91 eligible cases were chosen from them using the method of polymerase chain reaction （PCR） to amplify ureC. The subtypes of cagA, iceA genes were detected by PCR and agarose gel electrophoresis. Relationship between different combinations of genotypes and disease was analyzed using ;（2 test and Fisher exact test. RESULTS： The cagA-positive rate was 79.1%, and the overall detection rate of iceA was 75.82%, in which the detection rate of iceA1 was 50.5% and that of iceA2 was 38.5%. The positive rate of cagA＋/iceA1＋ was higher than that of other groups. Each gene alone or genes in different combinations didn＇t show any statistical signifi- cance in clinical outcomes. The relevance existed in iceA and cagA. 229 bp, 334 bp, 439 bp, 549 bp and 229 bp＋334 bp fragments of the genes were found in iceA2. CONCLUSION： cagA＋/iceA1＋ is the dominant genotype of H pylori in Xi＇an area. Neither a single gene nor the combination of cagA, iceA1, iceA2 are helpful in predicting the clinical outcome of H pylori infection. We may find synergy with the iceA and cagA. iceA2 shows greater variability in Xi＇an area.

关 键 词：幽门螺杆菌 CAGA ICEA 聚合酶链反应 

分 类 号：R573[医药卫生—消化系统]