5-氮-2-脱氧胞苷联合曲古抑菌素A对人胃癌细胞系MGC-803生物学行为的影响  

Effects of 5-aza-2-deoxycytidine and trichostatin A on biological behavior in MGC-803 cells

作　　者：郑向欣[1] 王建[1] 

机构地区：[1]徐州医学院第二附属医院普外科,江苏省徐州市221000

出　　处：《世界华人消化杂志》2009年第16期1655-1659,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨5-氮-2-脱氧胞苷(5-Aza-CdR)和曲古抑菌素A(TSA)对人胃癌细胞系MGC-803生物学行为的影响.方法:人胃癌细胞系MGC-803按常规培养于含100g/L小牛血清的RPMI1640培养液中.实验分为5组:(1)空白对照组:不含细胞的培养液;(2)阴性对照组:细胞只换液,不加药物;(3)5-Aza-CdR组:细胞接种24h后,加入10.0μmol/L的5-Aza-CdR;(4)TSA组:细胞接种24h后,加入300μg/LTSA;(5)5-Aza-CdR+TSA组:细胞接种24h后,加入10.0μmol/L5-Aza-CdR,24h后再加入300μg/LTSA.应用RT-PCR检测各组细胞培养72h后细胞Runx3 mRNA的表达,MTT比色法测定各组细胞分别培养24、48、72h后细胞增殖.结果:单独应用5-Aza-CdR和TSA作用于MGC-803细胞72h后,Runx3 mRNA相对表达量增加,联合用药组分别与5-Aza-CdR组及TSA组相比,差异有统计学意义(0.883±0.025 vs 0.760±0.286,0.735±0.018,均P<0.05).细胞生长抑制率在同一组别随着药物作用时间的延长而增加,并且呈正相关(r=0.738,P<0.05);而在同一时间不同组别比较联合用药组细胞生长抑制率较单用药物组明显增加(24h:57.3% vs 40.4%,39.0%;48h:70.0% vs 56.0%,51.3%;72h:86.3% vs 68.0%,65.8%,均P<0.05).细胞Runx3 mRNA的相对表达量及生长抑制率在药物组与对照组间比较,差异均有统计学意义(P<0.05).结论:与单用5-Aza-CdR或TSA相比,联合应用2种药物更显著的增强Runx3 mRNA的表达,抑制细胞的增殖.AIM： To explore the effects of 5-aza-2- deoxycytidine （5-Aza-CdR） and trichostatin A （TSA） on biological behavior in MGC-803 Cells. METHODS： MGC-803 cells were cultured in RPMI 1640 culture medium containing 100 g/L fetal calf serum. The cells in experiment were at logarithmic phase, and the number of viable cells was 95%-98%. The trial consisted of five groups. （1） Blank control group： there was only culture medium and no cells or drugs; （2） Negative control group： there were cells, culture medium and no drugs; （3） 5-Aza-CdR group： 24 h after inoculation, MGC-803 cells were treated with 10.0 μmol/L 5-Aza-CdR; （4） TSA group： 24 h after inoculation, MGC-803 cells were treated with 300 μg/L TSA;. （5） 5-Aza-CdR＋TSA group： 24 h after inoculation, MGC-803 cells were treated with 10.0 μmol/L 5-Aza-CdR, and thentreated with 300 μg/L TSA after 24 h. After MGC-803 cells were treated with different concentrations of 5-Aza-CdR and TSA for different cultural time, the expression of Runx3 mRNA in different groups for 72 h was detected by RT-PCR, and the cell proliferation in different groups for 24, 48, 72 h was determined using MTT colorimetry. RESULTS： When MGC-803 cells were treated with 5-Aza-CdR and TSA alone for 72 h, the rel- ative expression of Runx3 mRNA was increased. It was significantly increased in combination group, and compared with 5-Aza-CdR group and TSA group （0.883 ± 0.025 vs 0.760 ± 0.286, 0.735 ± 0.018, both P 〈 0.05）. The cell growth inhibition rate increased with the time prolong- ing in the same group and there was a positive relationship （r = 0.738, P 〈 0.05）. The cell growth inhibition rate increased more significantly in combination group compared with 5-Aza-CdR group and TSA group in the same time （24 h： 57.3% vs 40.4%, 39.0%; 48 h： 70.0% vs 56.0%, 51.3%; 72 h： 86.3% vs 68.0%, 65.8%, all P 〈 0.05）. There were significant differences between drug group and control group in relative expression of Runx3 mRNA and cell growth i

关 键 词：5-氮一2一脱氧胞苷 曲古抑菌素A.胃癌细胞 RUNX3 甲基化 

分 类 号：R735.2[医药卫生—肿瘤]