HBx上调HepG2细胞DNA修复酶hMTH1表达的意义  

Significance of HBx gene up-regulated expression of DNA repair enzyme hMTH1 in the HepG2 cells

作　　者：郭晓榕[1] 程斌[1] 郑要初[1] 王颖[1] 王凡[1] 夏秀梅[1] 黎培员[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院消化内科,湖北省武汉市430030

出　　处：《世界华人消化杂志》2009年第16期1660-1664,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30570821~~

摘　　要：目的:探讨DNA氧化损伤修复酶hMTH1在HBx致肝细胞癌发生机制中的作用.方法:应用HPLC/ECD法检测稳定表达HBx的转基因细胞HepG2/HBx及其对照组HepG2与HepG2/pcDNA3.1细胞中的8-OHdG的含量;并以β-actin为内对照,应用RT-实时PCR定量检测各组细胞中可水解8-OHdG的DNA修复酶hMTH1的表达.结果:8-OHdG在HepG2/HBx细胞中的含量(fmol8-OHdG/μgDNA)显著高于对照组HepG2和HepG2/pDNA3.1细胞(36.5±6.25vs8.52±1.65,9.12±2.69,均P<0.05).DNA修复酶hMTH1在HepG2/HBx细胞中的表达较两对照组细胞明显增高(1.213±0.100vs0.087±0.026,0.112±0.052hMTH1/β-actin mRNA×100,均P<0.05).结论:HBx可能通过诱导氧化应激增加HepG2细胞内DNA氧化损伤产物8-OHdG的含量,从而反应性上调DNA修复酶hMTH1的表达.AIM： To explore the role of oxidative DNA damage repair enzyme hMTH1 in the HBxinduced hepatocellular carcinoma. METHODS： 8-OHdG levels were determined using HPLC/ECD in the HepG2/HBx and in HepG2 and HepG2/pcDNA3.1 of the control cells. Using the β-actin as the interior control, real-time quantitative polymerase chain reaction （qPCR） was employed to examine the expression of DNA repair enzyme hMTH1 of hydrolyze 8-OHdG. RESULTS： The 8-OHdG level was significantly higher in the HepG2/HBx than in HepG2 andHepG2/pcDNA3.1 （36.5± 6.25 vs 8.52 ± 1.65, 9.12 ± 2.69 fmol 8-OHdG/μg DNA, both P 〈 0.05）, and the expression of DNA repair enzyme hMTH1 mRNA was significantly higher than the control cells （1.213 ± 0.100 vs 0.087 ±0.026, 0.112 ± 0.052 hMTH1/β-actin mRNA×100, both P 〈 0.05）. CONCLUSION： HBx gene may increase the level of oxidative DNA-adduct 8-OHdG by promot- ing the oxidative stress in HepG2 cells, thus reactivity increases the expression of DNA repair enzyme hMTH1.

关 键 词：乙型肝炎病毒X基因 HEPG2细胞 HPLC/ECD hMTH1 

分 类 号：R735.7[医药卫生—肿瘤] R734.2[医药卫生—临床医学]