生物钟基因hPer2在人结直肠癌中的表达及其临床意义  

Clock gene hPer2 expression and its clinical significance in human colorectal carcinoma

作　　者：王雅平[1] 华鲁纯[1] 蒿汉坤[1] 陈宗祐[1] 

机构地区：[1]复旦大学附属华山医院普外科,上海200040

出　　处：《中华实验外科杂志》2009年第8期1034-1036,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测hPer2蛋白在人结直肠癌中的表达，探讨其临床意义。方法取人结直肠癌及癌旁组织各38例，以免疫组织化学方法检测其中hPer2蛋白的表达，并分析其表达与临床及病理常用指标的关系。结果在结直肠癌及癌旁正常黏膜细胞中hPer2均有表达，阳性表达率分别为81．6％（31／38）及97．4％（37／38），两者间差异有统计学意义（P〈0．05）。以癌旁组织为参照，hPer2在癌组织中表达下调者占63．1％（24／38），表达上调者占5．3％（2／38），无明显区别者占31．6％（12／38），其差异有统计学意义（P〈0．01）。hPer2表达失调与结直肠癌患者年龄≤50岁、肿瘤组织学分级高、浸润深度深、有淋巴结转移、TNM分期晚相关（P〈0．05）。结论hPer2在人结直肠癌中表达下调。hPer2表达的失调甚至缺失可能与结直肠癌的生长、浸润、转移相关。Objective To detect the expression of a circadian gene, hPer2, in human colorectal carcinoma, and analyze its relation to clinico-pathological data. Methods The expression of hPer2 was detected in 38 paired colorectal carcinoma tissues and surrounding non-cancerous tissues by immunohistochemical staining. The relationship between hPer2 and several clinicopathological parameters was analyzed. Results hPer2 protein was positively stained in 81.6% （31/38） of colorectal carcinoma tissues and 97.4% （37/38） of surrounding non-cancerous tissues （ P 〈 0.05 ）. Compared with the surrounding non-cancerous tissues ,the expression of hPer2 protein was decreased in 24 cases （63.1% ）, and increased in 2 cases （5.3%）, while no obvious difference was found in 12 cases （31.6% ） （P 〈 0.01 ）. Low expression of hPer2 protein in cancerous cells was correlated with age （ ~〈50 years）, pathological differentiation, depth of tumor invasion,lymph nodes metastasis, and TNM stage of colorectal carcinoma （P 〈 0.05 ）. Conclusion The expression hPer2 protein was decreased in human colorectal carcinoma. There was a positive correlation between the down-regulation of hPer2 protein with the development, invasion, and metastasis in colorectal carcinoma patients.

关 键 词：结直肠肿瘤 hPer2蛋白 病理学 临床 

分 类 号：R735.34[医药卫生—肿瘤]