Testin基因在结直肠癌组织中的表达及临床意义  

Expression of Testin in human colorectal carcinoma and its clinical significance

作　　者：龙结根[1] 张才全[1] 赵林[1] 

机构地区：[1]重庆医科大学附属第一医院胃肠外科,重庆400016

出　　处：《中国癌症杂志》2009年第6期428-432,共5页China Oncology

摘　　要：背景与目的:人类Testin基因是一个定位于染色体7q31.2的候选抑癌基因。本实验研究人结直肠癌中候选抑癌基因Testin的表达,并分析其与结直肠癌临床病理特征的关系。方法:应用RT-PCR和免疫组化SP法检测60例患者结直肠癌组织及对应正常组织(距肿瘤15cm以上)中Testin的表达。同时分析Testin的表达与患者临床病理特征的关系。结果:结直肠癌组织中TestinmRNA的表达水平显著低于正常组织(P<O.O1);免疫组化结果显示Testin在31.7%(19/60)的结直肠癌组织中呈阳性,而在正常组织中有86.7%(52/60)阳性(P<0.01)。统计分析结果发现,Testin蛋白与mRNA的表达与结直肠癌患者的性别、年龄、肿瘤部位无关(P>0.05),而与肿瘤浸润深度、分化程度、Dukes分期、T分期、有无淋巴结转移和远处转移有关(P<0.05)。浸润越深、分化程度越低、Dukes分期越晚、T分期越晚、有淋巴结转移和有远处转移的癌组织的Testin表达明显降低;Testin蛋白与mRNA的表达呈显著正相关(r=0.75,P<0.O5)。结论:Testin在结直肠癌中的表达量显著下降,可能与结直肠癌的浸润、分化和转移相关,提示Testin基因表达下调可能对结直肠癌的发生、发展具有重要作用。可能成为判断结肠癌预后和浸润转移的生物学指标。Background and purpose： The Testin gene is a candidate tumor suppressor gene located at 7q31.2. Our purpose was to investigate the expression of Testin gene, and its possible relationship with the clinicopathological features of human colorectal carcinoma. Methods： Semi-quantitative reverse transcriptionpolymerase chain reaction （RT-PCR） and immunohistochemistry methods were used to investigate the expression of Testin in 60 specimens of colorectal carcinomas and corresponding adjacent normal mucosal tissues. The correlations of Testin to clinicopathologic features of colorectal carcinoma were analyzed. Results： The mRNA level of Testin was down-regulated in colorectal carcinomas. The positive rate of Testin protein was significantly lower than the paracancerous mucosal normal tissues （P〈0.01） . The expression of Testin protein and mRNA was correlated with infiltration, differentiation, Dukes stage, T stage,lymph node metastasis and distant metastasis （P〈0.05）, but not with patients＇ gender, age, site of cancer （P〉0.05）. The expression of Testin protein was lower in the tissues which were deeper infiltrated, poorer differentiated, advanced in Dukes stage and T stage, or with lymph node or remote metastasis. The expression of Testin mRNA was positively associated with that of Testin protein（r=0.75,P〈0.05）. Conclusion： The expression level of Testin is significantly down-regulated in colorectal carcinomas, which may be associated with the degree of infiltration, differentiation and metastasis of colorectal carcinoma. It suggests that Testin may be a valuable marker for assessing the prognosis and metastasis of colorectal carcinoma.

关 键 词：结直肠癌 Testin基因 逆转录-聚合酶链反应 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤] R730.21[医药卫生—临床医学]