藤茶多糖AGP-3的分离纯化与结构的初步鉴定  

Isolation and Purification and Preliminary Structure Identification of Polysaccharide Fraction AGP-3 from Ampelopsis grossedentata

作　　者：罗祖友[1,2] 陈根洪[2] 郑小江[1] 吴谋成[3] 

机构地区：[1]湖北民族学院湖北省生物资源保护与利用重点实验室 [2]湖北民族学院生物科学与技术学院 [3]华中农业大学食品科技学院

出　　处：《时珍国医国药》2009年第7期1707-1709,共3页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省自然科学基金(No.2006ABA036);国家民委部分资助项目(No.08HB04)

摘　　要：目的对藤茶水溶性多糖进行分离纯化得到AGP-3组分,并对其结构进行初步的鉴定,为藤茶多糖结构与生物活性关系的研究奠定基础。方法采用DEAE-SephadexA-25、SephadexG-200和HPLC对藤茶多糖分离纯化,并通过IR、GC、1HNMR和13CNMR分析对藤茶多糖AGP-3组分的结构进行了初步鉴定。结果AGP-3重均分子量(Mw)为1.02×105Da,含中性糖质量分数57.6%、糖醛酸32.3%、蛋白质3.5%。结论藤茶多糖分离组分AGP-3为一种含蛋白质的复合多糖。Objective To provide a basis for the research about the relation between the structure and its bioactivities of polysaccharides from Ampelopsis grossedentata. The polysaccharide fraction AGP - 3 was obtained and analyzed preliminarily after water - soluble polysaccharides from Ampelopsis grossedentata being isolated and purified. Methods The water - soluble polysaccharides from Ampelopsis grossedentata were isolated and purified with DEAE - Sephadex A - 25, Sephadex G - 200 and HPLC, and the structure of AGP- 3 was analyzed preliminarily by IR, GC, IHNMR and 13CNMR. Results The molecular averageweight （MW） of AGP - 3 was 1.02× 105 Da, containing 57.6% neutral sugar, 32.3% uronic acid and 3.5% protein. Conclusion The polysaccharide fraction AGP - 3 from Ampelopsis grossedentata is a kind of conjugated polysaccharide containing protein.

关 键 词：藤茶 多糖 分离纯化 结构鉴定 

分 类 号：R284.2[医药卫生—中药学]