激活转录因子3的过表达对食管癌细胞生长的抑制作用  

Over-expression of ATF3 Inhibits the Proliferation of Esophageal Squamous Carcinoma Cells

作　　者：谢仰民[1] 谢剑君[2] 周飞[1,2] 侯健[2] 曹君君[1] 许丽艳[3] 李恩民[2] 

机构地区：[1]汕头大学医学院实验动物中心,广东汕头515041 [2]汕头大学医学院生物化学与分子生物学教研室,广东汕头515041 [3]汕头大学医学院肿瘤病理研究室,广东汕头515041

出　　处：《癌变．畸变．突变》2009年第4期258-262,267,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广东省科技厅计划项目(2004B60301006)

摘　　要：背景与目的:激活转录因子3(activating transcription factor 3,ATF3)在食管癌中表达异常下调,但其功能仍不清楚。本研究拟探讨ATF3在食管癌细胞过表达对癌细胞生长及裸鼠成瘤的影响。材料与方法:利用分子克隆技术将ATF3基因完整编码区克隆至真核细胞表达载体pcDNA3中,获得重组表达质粒;将该表达质粒转染食管癌EC109细胞并用G418筛选稳定表达的细胞克隆;用Western blot检测ATF3的过表达效果;对ATF3过表达的细胞与相应对照细胞进行细胞克隆形成实验和裸鼠体内成瘤实验,以分析ATF3过表达对食管癌细胞生长的影响。结果:ATF3的过表达在体外可以降低食管癌细胞的克隆形成能力;在体内可以抑制食管癌细胞在雌鼠中的成瘤能力,但对雄鼠中的成瘤能力没有明显影响。结论:ATF3的过表达可以抑制食管癌细胞的生长,ATF3可能在食管癌的发生发展中发挥重要作用。BACKGROUND AND AIM： ATF3 was down-regulated in esophageal squamous cell carcinoma （ESCC）, but the roles of ATF3 in ESCC cells still remained unclear. The purpose of this study was to explore the effect of ATF3 on the proliferation of ESCC cells. MATERIALS AND METHODS： The recombinant expressing plasmid was constructed by inserting the full coding sequence of ATF3 gene into the eukaryotic expressing vector pcDNA3. Then, the expressing plasmid was stably transfected into EC109 cells, an ESCC cell line, and the over-expressing ATF3 cell clones were obtained. Colony formation assay and tumor formation assay in nude mice were used to explore the effect of ATF3 over-expression on the proliferation of ESCC cells. RESULTS： With the over-expression of ATF3, the colony formation ability of EC109 cells was decreased and the tumor formation of EC109 cells in female mice was inhibited. CONCLUSION： Over-expression of ATF3 could inhibit the proliferation of ESCC ceils and ATF3 may play important roles in the progression of ESCC.

关 键 词：食管癌 激活转录因子3 细胞生长 

分 类 号：Q71[生物学—分子生物学] R735.1[医药卫生—肿瘤]